Rabbit anti-Human Paxillin Polyclonal Antibody | anti-PXN antibody

Paxillin (Y118) (PXN) (APC)

Cat. Num. AAA6492282

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Paxillin; Polyclonal Antibody; Paxillin (Y118) (PXN) (APC); anti-PXN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Paxillin.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 557

• Applicable Applications for anti-PXN antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 94-125 of human Paxillin, UniProt Accession #P49023.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PXN (arrow) in 293 cell lysates (2 ug/lane) using P3113-91G either nontransfected (Lane 1) or transiently transfected with PXN gene (Lane 2).)

Testing Data

(All lanes: P3113-91G (1:1000) Lane 1: A431 whole cell lysate Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 65kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with P3113-91G, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of P3113-91G for immunohistochemistry.)

Product Categories/Family for anti-PXN antibody

Antibodies; Paxillin

NCBI and Uniprot Product Information

• NCBI GI #: 170932516
• NCBI GeneID: 5829
• NCBI Accession #: NP_002850
• NCBI GenBank Nucleotide #: NP_002850.2
• UniProt Accession #: P49023
• Molecular Weight: 64.533
• NCBI Official Full Name: paxillin isoform 1
• NCBI Official Synonym Full Names: paxillin
• NCBI Official Symbol: PXN
• NCBI Protein Information: paxillin
• UniProt Protein Name: Paxillin
• Protein Family: Paxillin
• UniProt Gene Name: PXN
• UniProt Entry Name: PAXI_HUMAN

NCBI Description

This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]

Uniprot Description

PXN: a multi-domain cytoskeletal protein involved in actin-membrane attachment that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions. Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the Crk SH2 domain. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.Three alternatively-spliced isoforms have been described. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis; Cell adhesion; Cytoskeletal

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; microtubule associated complex; focal adhesion; lamellipodium; cytoplasm; plasma membrane; stress fiber; cell cortex; cytosol

Molecular Function: integrin binding; protein binding; zinc ion binding; beta-catenin binding; BH4 domain binding; protein kinase binding; vinculin binding

Biological Process: epidermal growth factor receptor signaling pathway; lamellipodium biogenesis; focal adhesion formation; integrin-mediated signaling pathway; peptidyl-tyrosine phosphorylation; activation of MAPK activity; signal complex assembly; cytoskeleton organization and biogenesis; signal transduction; regulation of cell shape; branching morphogenesis of a tube; muscle contraction; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; cell adhesion