Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using Paxlllln (Phospho-Tyr88) antibody)
Rabbit Paxillin Polyclonal Antibody | anti-PXN antibody
Paxillin antibody
Gene Names
PXN; TGFB1I1
Applications
Immunofluorescence, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
Paxillin; Polyclonal Antibody; Paxillin antibody; Rabbit polyclonal Paxillin antibody; Polyclonal Paxillin antibody; Anti-Paxillin antibody; Phospho Tyr88 antibody; PXN antibody; anti-PXN antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
559
Applicable Applications for anti-PXN antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF: 1:100-1:200
WB: 1:500-1:1000
WB: 1:500-1:1000
Immunogen
Paxillin antibody was raised in Rabbit using the peptide sequence around phosphorylation site of tyrosine 88 (P-V-Y(p)-G-S) derived from Human Paxillin as the immunogen.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using Paxlllln (Phospho-Tyr88) antibody)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using Paxlllln (Phospho-Tyr88) antibody)
Related Product Information for anti-PXN antibody
Paxillin is a signal transduction adaptor protein discovered in 1990 in the laboratory of Keith Burridge and should not be confused with the neurotoxin paxilline. The C-terminal region of paxillin contains four LIM domains that target paxillin to focal adhesions, it is presumed through a direct association with the cytoplasmic tail of beta-integrin. The N-terminal region of paxillin is rich in protein protein interaction sites. The proteins that bind to paxillin are diverse and include protein tyrosine kinases, such as Src and FAK, structural proteins, such as vinculin and actopaxin, and regulators of actin organization, such as COOL/PIX and PKL/GIT.
Product Categories/Family for anti-PXN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61,243 Da
NCBI Official Full Name
paxillin
NCBI Official Synonym Full Names
paxillin
NCBI Official Symbol
PXN
NCBI Official Synonym Symbols
TGFB1I1
NCBI Protein Information
paxillin
UniProt Protein Name
Paxillin
Protein Family
UniProt Gene Name
PXN
Uniprot Description
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of v-CRK.
Research Articles on PXN
Similar Products
Product Notes
The PXN pxn (Catalog #AAA5309631) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Paxillin can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). IF: 1:100-1:200 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the PXN pxn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Paxillin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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