Testing Data
(Fetal Mouse brain lysate was resolved by electrophoresis, transferred to PVDF and probed with P3113-84D3 (1:2000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PAX6 (~48kD).)
Rabbit PAX6 Polyclonal Antibody | anti-PAX6 antibody
PAX6 (Paired Box 6, AN, AN2, Aniridia Type II Protein, D11S812E, MGC17209, MGDA, Oculorhombin, Paired Box Protein Pax-6, WAGR) (PE)
Gene Names
PAX6; AN; AN2; MGDA; WAGR; D11S812E
Reactivity
Mouse, Chicken, Rat
Applications
Immunocytochemistry, Western Blot
Purity
Purified by peptide affinity chromatography.
Synonyms
PAX6; Polyclonal Antibody; PAX6 (Paired Box 6; AN; AN2; Aniridia Type II Protein; D11S812E; MGC17209; MGDA; Oculorhombin; Paired Box Protein Pax-6; WAGR) (PE); anti-PAX6 antibody
Host
Rabbit
Reactivity
Mouse, Chicken, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse PAX6. Species Crossreactivity: chicken and rat. Does not react with human PAX6. Species Sequence Homology: bovine and canine.
Purity/Purification
Purified by peptide affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-PAX6 antibody
Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
ICC: 1:50-1:1000 used on chicken spinal cord.
WB: 0.5ug/ml detected PAX6 on 10ug of mouse and rat brain lysates.
Applications are based on unconjugated antibody.
WB: 0.5ug/ml detected PAX6 on 10ug of mouse and rat brain lysates.
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to the C-Terminus region of mouse PAX6.
Conjugate
PE
Positive Control
Fetal mouse and rat brain lysates
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Testing Data
(Fetal Mouse brain lysate was resolved by electrophoresis, transferred to PVDF and probed with P3113-84D3 (1:2000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PAX6 (~48kD).)
Testing Data
(Fetal Mouse brain lysate was resolved by electrophoresis, transferred to PVDF and probed with P3113-84D3 (1:2000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PAX6 (~48kD).)
Testing Data
(Working dilutions of 1:50-1:1000 of P3113-84D3 was used to detect the protein in chicken spinal cord.)
Testing Data
(Working dilutions of 1:50-1:1000 of P3113-84D3 was used to detect the protein in chicken spinal cord.)
Product Categories/Family for anti-PAX6 antibody
References
1. Hallbook, F et al. (2008). Dev. Biology. 8:46. 2. Callaerts P, et al. (1997). Annu. Rev. Neurosci. 20: 483-532. 3. Prosser, J., van Heyningen, V. (1998). Hum. Mutat. 11 (2): 93-108. 4. Glaser, T., et al. (1994). Nat. Genet. 2 (3): 232-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
422
NCBI Official Full Name
paired box protein Pax-6 isoform a
NCBI Official Synonym Full Names
paired box 6
NCBI Official Symbol
PAX6
NCBI Official Synonym Symbols
AN; AN2; MGDA; WAGR; D11S812E
NCBI Protein Information
paired box protein Pax-6; oculorhombin; aniridia type II protein; paired box homeotic gene-6
UniProt Protein Name
Paired box protein Pax-6
Protein Family
UniProt Gene Name
PAX6
UniProt Synonym Gene Names
AN2
UniProt Entry Name
PAX6_HUMAN
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Product Notes
The PAX6 pax6 (Catalog #AAA6492258) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAX6 (Paired Box 6, AN, AN2, Aniridia Type II Protein, D11S812E, MGC17209, MGDA, Oculorhombin, Paired Box Protein Pax-6, WAGR) (PE) reacts with Mouse, Chicken, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PAX6 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB). ICC: 1:50-1:1000 used on chicken spinal cord. WB: 0.5ug/ml detected PAX6 on 10ug of mouse and rat brain lysates. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PAX6 pax6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAX6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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