Rabbit anti-Mouse Pax3 Polyclonal Antibody | anti-PAX3 antibody

Pax3

Cat. Num. AAA613683

• Gene Names: PAX3; WS1; WS3; CDHS; HUP2; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778
• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Purity: Serum; Serum
• Synonyms: Pax3; Polyclonal Antibody; Anti -Pax3; anti-PAX3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the mouse Pax3 protein at ~53kD in Pax3-MSCV transduced NIH-3T3 cell lysates on a Western Blot assay. An untransduced NIH-3T3 cell line did not exhibit the target ~53kD band. However, a strong non-specific band at ~70kD and several other faint bands were observed in both the positive and negative control cell lines. Has also been used in immunofluorescence assays using Pax3-FKHR infected NIH-3T3 cells that were fixed in 2% paraformaldehyde and permeabilized in 2% paraformaldehyde containing 1% Triton X-100. Species sequence homology: human and chicken.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied a liquid, 0.1% sodium azide.

• Applicable Applications for anti-PAX3 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Immunofluorescence: 1:100-1:500 Pax3-FKHR infected NIH-3T3 cells were fixed in 2% paraformaldehyde and permeabilized in 2% paraformaldehyde containing 1% Triton X-100; Western Blot: 1:100-1:500; Immunoprecipitation: 1:100-1:500; Immunohistochemistry (Frozen and FFPE): 1:100-1:500. Use with formalin-fixed, parrafin-embedded mouse tissue sections, requires heat induced epitope retrieval (HEIR) using citrate buffer, pH 6.0 for 10 minutes.
• Immunogen: His6-tagged recombinant protein derived from a portion of the N-terminus of the mouse Pax3 sequence, encompassing the paired box and homeobox domains. Species sequence homology: human and chicken.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Pax3-MSCV transduced NIH 3T3 cell lysates using MBS613683.)

Related Product Information for anti-PAX3 antibody

Pax3 is a transcription factor that is required by pre-migratory neural crest cells that give rise to the peripheral nervous system, melanocytes, some vascular smooth muscle, and numerous other derivatives, and is localized in the dorsal neural tube, and in myogenic progenitors in the presomitic mesoderm and the hypaxial somites.1 Repressors of Pax3 transcriptional activity include the transcriptional co-repressor hDaxx, the retinoblastoma protein (Rb) and Calmyrin. The Pax3-FKHR fusion protein is formed due to chromosomal translocation and induces morphological change and enhances cellular proliferation and invasion in alveolar rhabdomyosarcoma (ARMS) development.2 Pax3 mutations help cause Waardenburg Syndrome, which is an auditory-pigmentary syndrome caused by a deficiency of melanocytes and other neural crest-derived cells.3 Pax3 is also involved in the development and progression of malignant melanoma.

Product Categories/Family for anti-PAX3 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 31563340
• NCBI GeneID: 5077
• NCBI Accession #: NP_852122.1
• NCBI GenBank Nucleotide #: NM_181457.3
• UniProt Accession #: P23760; Q16448; Q53T90; Q6GSJ9; Q12777; Q494Z3; Q494Z4; Q86UQ2; Q86UQ3
• Molecular Weight: 52,968 Da
• NCBI Official Full Name: paired box protein Pax-3 isoform PAX3
• NCBI Official Synonym Full Names: paired box 3
• NCBI Official Symbol: PAX3
• NCBI Official Synonym Symbols: WS1; WS3; CDHS; HUP2; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778
• NCBI Protein Information: paired box protein Pax-3; OTTHUMP00000164207; OTTHUMP00000164208; OTTHUMP00000164209; OTTHUMP00000202744; OTTHUMP00000202745; OTTHUMP00000202746; OTTHUMP00000205137; OTTHUMP00000224703; paired domain gene 3; paired domain gene HuP2; paired box homeotic gene 3
• UniProt Protein Name: Paired box protein Pax-3
• Protein Family: Paired box protein
• UniProt Gene Name: PAX3
• UniProt Synonym Gene Names: HUP2
• UniProt Entry Name: PAX3_HUMAN

NCBI Description

This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq]

Uniprot Description

PAX3: Probable transcription factor associated with development of alveolar rhabdomyosarcoma. Can bind to DNA as a heterodimer with PAX7. Interacts with DAXX. Belongs to the paired homeobox family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; sequence-specific DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; muscle development; apoptosis; heart development; positive regulation of transcription, DNA-dependent; pigmentation during development; neuron fate commitment; negative regulation of transcription from RNA polymerase II promoter; cell proliferation; organ morphogenesis; sensory perception of sound; neural tube closure; positive regulation of cell proliferation; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of somitogenesis; neural crest cell migration

Disease: Waardenburg Syndrome, Type 3; Craniofacial-deafness-hand Syndrome; Rhabdomyosarcoma 2; Waardenburg Syndrome, Type 1