Western Blot (WB)
(Anti-PAX2 rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:44 kDa Observed band size:44 kDa)
Rabbit anti-Human, Mouse PAX2 Polyclonal Antibody | anti-PAX2 antibody
Anti-PAX2 Antibody, Rabbit Polyclonal
IHC-P: 1:500-1:2000
ICC/IF: 1:100-1:500
IP: 0.5-2uL/mg of lysate
Western Blot (WB)
(Anti-PAX2 rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:44 kDa Observed band size:44 kDa)
Western Blot (WB)
(Anti-PAX2 rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:44 kDa Observed band size:44 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human PAX2 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PAX2 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PAX2 in human renal carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PAX2 in human renal carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PAX2 in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PAX2 in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of PAX2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PAX2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of PAX2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PAX2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Immunoprecipitation (IP)
(CBX4 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 4 uL anti-CBX4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CBX4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa Observed band size :45 kDa)
Immunoprecipitation (IP)
(CBX4 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 4 uL anti-CBX4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CBX4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa Observed band size :45 kDa)
NCBI and Uniprot Product Information
NCBI Description
PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor suppressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Uniprot Description
PAX2: Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS. Interacts with ELGN3; the interaction targets PAX2 for destruction. Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system. 4 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 10q24
Cellular Component: protein complex; lysosome; microtubule organizing center; nucleus
Molecular Function: protein binding; DNA binding; superoxide-generating NADPH oxidase activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; negative regulation of cytolysis; optic nerve structural organization; positive regulation of transcription, DNA-dependent; cell fate determination; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; pronephros development; glial cell differentiation; vestibulocochlear nerve formation; visual perception; pancreas development; negative regulation of programmed cell death; protein kinase B signaling cascade; brain morphogenesis; mesonephros development; aging; response to nutrient levels; inner ear morphogenesis; camera-type eye development; transcription, DNA-dependent; optic cup morphogenesis involved in camera-type eye development; stem cell differentiation; optic nerve morphogenesis; axonogenesis; optic nerve development; ureteric bud branching; neural tube closure; mesodermal cell fate specification; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; urogenital system development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis
Disease: Papillorenal Syndrome; Renal Hypodysplasia/aplasia 1; Focal Segmental Glomerulosclerosis 7
Research Articles on PAX2
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Product Notes
The PAX2 pax2 (Catalog #AAA8101454) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PAX2 Antibody, Rabbit Polyclonal reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PAX2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:500-1:2000 ICC/IF: 1:100-1:500 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the PAX2 pax2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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