Rabbit anti-Human PAX2 Polyclonal Antibody | anti-PAX2 antibody

PAX2, ID (PAX2, Paired box protein Pax-2)

Cat. Num. AAA647049

• Gene Names: PAX2; PAPRS
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PAX2; Polyclonal Antibody; ID (PAX2; Paired box protein Pax-2); Anti -PAX2; anti-PAX2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PAX2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: PAX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-216 amino acids from the Central region of human PAX2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PAX2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PAX2 antibody detected the PAX2 protein (arrow).)

Related Product Information for anti-PAX2 antibody

PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor supressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants.

Product Categories/Family for anti-PAX2 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 34878716
• NCBI GeneID: 5076
• NCBI Accession #: NP_003980.2
• NCBI GenBank Nucleotide #: NM_003989.3
• UniProt Accession #: Q02962; Q15105; Q15110; Q15837; Q5SZP2; Q5SZP3
• Molecular Weight: 44,706 Da
• NCBI Official Full Name: paired box protein Pax-2 isoform d
• NCBI Official Synonym Full Names: paired box 2
• NCBI Official Symbol: PAX2
• NCBI Official Synonym Symbols: PAPRS
• NCBI Protein Information: paired box protein Pax-2; paired box homeotic gene 2
• UniProt Protein Name: Paired box protein Pax-2
• Protein Family: Paired box protein
• UniProt Gene Name: PAX2
• UniProt Entry Name: PAX2_HUMAN

NCBI Description

PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor supressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS.

Subunit structure: Interacts with ELGN3; the interaction targets PAX2 for destruction. Ref.8

Subcellular location: Nucleus.

Tissue specificity: Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.

Developmental stage: Mainly in fetal kidney and juvenile nephrogenic rests.

Involvement in disease: Renal-coloboma syndrome (RCS) [MIM:120330]: An autosomal dominant disorder characterized by both ocular and renal anomalies, but may also include vesicoureteral reflux, high frequency hearing loss, central nervous system anomalies, and/or genital anomalies. Eye anomalies in this disorder consist of a wide and sometimes excavated dysplastic optic disk with the emergence of the retinal vessels from the periphery of the disk, designated optic nerve coloboma or 'morning glory' anomaly. Associated findings may include a small corneal diameter, retinal coloboma, scleral staphyloma, optic nerve cyst, microphthalmia, and pigmentary macular dysplasia. The kidneys are small and abnormally formed (renal hypodysplasia), and have fewer than the normal number of glomeruli, which are enlarged (oligomeganephronia). These ocular and renal anomalies result in decreased visual acuity and retinal detachment, as well as hypertension, proteinuria, and renal insufficiency that frequently progresses to end-stage renal disease.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10 Ref.13 Ref.14 Ref.15Defects in PAX2 can be responsible for isolated renal hypodysplasia and oligomeganephronia (OMN). This is a rare congenital and usually sporadic anomaly characterized by bilateral renal hypoplasia, with a reduced number of enlarged nephrons and without urinary tract abnormalities. Ref.12

Sequence similarities: Contains 1 paired domain.