Goat anti-Human PARP3 Polyclonal Antibody | anti-PARP3 antibody

PARP3, ID (Poly [ADP-ribose] Polymerase 3, PARP-3, hPARP-3, IRT1, NAD(+) ADP-ribosyltransferase 3, ADPRT-3, Poly[ADP-ribose] Synthase 3, pADPRT-3, ADPRT3, ADPRTL3)

Cat. Num. AAA648982

• Gene Names: PARP3; IRT1; ARTD3; ADPRT3; ADPRTL2; ADPRTL3; PADPRT-3
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PARP3; Polyclonal Antibody; ID (Poly [ADP-ribose] Polymerase 3; PARP-3; hPARP-3; IRT1; NAD(+) ADP-ribosyltransferase 3; ADPRT-3; Poly[ADP-ribose] Synthase 3; pADPRT-3; ADPRT3; ADPRTL3); Anti -PARP3; anti-PARP3 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human PARP3.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-PARP3 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:2000; Immunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/ml
• Immunogen: Synthetic peptide YEDYNCTLNQTNIEN from an internal region of human PARP3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-PARP3 antibody

The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-PARP3 antibody

Antibodies; Abs to Enzymes; Polymerase

NCBI and Uniprot Product Information

• NCBI GI #: 158634482
• NCBI GeneID: 10039
• NCBI Accession #: NP_005476.3
• NCBI GenBank Nucleotide #: NM_005485.4
• UniProt Accession #: Q9Y6F1; Q8NER9; Q96CG2; Q9UG81
• Molecular Weight: 60,070 Da
• NCBI Official Full Name: poly
• NCBI Official Synonym Full Names: poly (ADP-ribose) polymerase family, member 3
• NCBI Official Symbol: PARP3
• NCBI Official Synonym Symbols: IRT1; ARTD3; ADPRT3; ADPRTL2; ADPRTL3; PADPRT-3
• NCBI Protein Information: poly [ADP-ribose] polymerase 3; poly [ADP-ribose] polymerase 3; ADPRT-3; poly[ADP-ribose] synthase 3; NAD+ ADP-ribosyltransferase 3; poly(ADP-ribose) synthetase-3; poly[ADP-ribose] synthetase 3; NAD(+) ADP-ribosyltransferase 3; ADP-ribosyltransferase diphtheria toxin-like 3; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 2; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 3
• UniProt Protein Name: Poly [ADP-ribose] polymerase 3
• Protein Family: Poly [ADP-ribose] polymerase
• UniProt Gene Name: PARP3
• UniProt Synonym Gene Names: ADPRT3; ADPRTL3; PARP-3; hPARP-3; ARTD3; ADPRT-3; pADPRT-3
• UniProt Entry Name: PARP3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. Binds DNA. May be involved in the regulation of PRC2 and PRC3 complex-dependent gene silencing. Ref.7

Catalytic activity: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.

Subunit structure: Interacts with PRKDC and PARP1. Interacts with XRCC5; the interaction is dependent on nucleic acids. Interacts with XRCC6; the interaction is dependent on nucleic acids. Interacts with EZH2, HDAC1, HDAC2, SUZ12, YY1, LRIG3 and LIG4. Ref.7

Subcellular location: Nucleus. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome › centriole. Note: Core component of the centrosome. Preferentially localized to the daughter centriole throughout the cell cycle. According to Ref.7, it is almost exclusively localized in the nucleus and appears in numerous small foci and a small number of larger foci whereas a centrosomal location has not been detected. Ref.3 Ref.7

Tissue specificity: Widely expressed; the highest levels are in the kidney, skeletal muscle, liver, heart and spleen; also detected in pancreas, lung, placenta, brain, leukocytes, colon, small intestine, ovary, testis, prostate and thymus.

Domain: According to Ref.2, the N-terminal domain (54 amino acids) of isoform 2 is responsible for its centrosomal localization. The C-terminal region contains the catalytic domain.

Post-translational modification: Auto-poly(ADP)-ribosylation.

Sequence similarities: Contains 1 PARP alpha-helical domain.Contains 1 PARP catalytic domain.