Rabbit anti-Human PARP3 Polyclonal Antibody | anti-PARP3 antibody

PARP3 Antibody

Cat. Num. AAA9630136

• Gene Names: PARP3; IRT1; ARTD3; ADPRT3; ADPRTL2; ADPRTL3; PADPRT-3
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PARP3; Polyclonal Antibody; PARP3 Antibody; ADP ribosyltransferase (NAD+; poly (ADP ribose); ADP ribosyltransferase diphtheria toxin like 3; ADPRT-3; ADPRT3; ADPRTL2; ADPRTL3; ARTD3; hPARP 3; hPARP-3; hPARP3; IRT 1; IRT1; NAD(+) ADP ribosyltransferase 3; NAD(+) ADP-ribosyltransferase 3; NAD+ ADP ribosyltransferase 3; pADPRT 3; pADPRT-3; pADPRT3; PARP 3; PARP-3; PARP3_HUMAN; Poly (ADP ribose) polymerase family; member 3; Poly (ADP ribose) synthetase 3; Poly [ADP-ribose] polymerase 3; Poly[ADP ribose] synthetase 3; Poly[ADP-ribose] synthase 3; anti-PARP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PARP3 Antibody detects endogenous levels of PARP3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PARP3 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human PARP3.
• Conjugation: Unconjugated
• Post Translational Modifications: Auto-mono-ADP-ribosylated.
• Subunit Structure: Interacts with PARP1; leading to activate PARP1 in absence of DNA. Interacts with PRKDC. Interacts with XRCC5/Ku80; the interaction is dependent on nucleic acids. Interacts with XRCC6/Ku70; the interaction is dependent on nucleic acids. Interacts with EZH2, HDAC1, HDAC2, SUZ12, YY1, LRIG3 and LIG4.
• Tissue Specificity: Widely expressed; the highest levels are in the kidney, skeletal muscle, liver, heart and spleen; also detected in pancreas, lung, placenta, brain, leukocytes, colon, small intestine, ovary, testis, prostate and thymus.
• Function: Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage. Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins. In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation. Associates with a number of DNA repair factors and is involved in the response to exogenous and endogenous DNA strand breaks. Together with APLF, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). Cooperates with the XRRC6-XRCC5 (Ku70-Ku80) heterodimer to limit end-resection thereby promoting accurate NHEJ. Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as XRRC5 and XRCC6. ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. In addition to proteins, also able to ADP-ribosylate DNA: mediates DNA mono-ADP-ribosylation of DNA strand break termini via covalent addition of a single ADP-ribose moiety to a 5'- or 3'-terminal phosphate residues in DNA containing multiple strand breaks. Acts as a negative regulator of immunoglobulin class switch recombination, probably by controlling the level of AICDA /AID on the chromatin (By similarity).
• Note: Almost exclusively localized in the nucleus and appears in numerous small foci and a small number of larger foci whereas a centrosomal location has not been detected (PubMed:16924674). In response to DNA damage, localizes to sites of double-strand break (PubMed:21270334). Preferentially localized to the daughter centriole (PubMed:10329013).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PARP3 antibody

Nucleus; Chromosome; Cytoplasm; Cytoskeleton; Microtubule organizing center; Centrosome; Centriole; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 158634478
• NCBI GeneID: 10039
• NCBI Accession #: NP_001003931.2
• NCBI GenBank Nucleotide #: NM_001003931.2
• UniProt Accession #: Q9Y6F1; Q8NER9; Q96CG2; Q9UG81
• Molecular Weight: 60,070 Da
• NCBI Official Full Name: poly
• NCBI Official Synonym Full Names: poly (ADP-ribose) polymerase family, member 3
• NCBI Official Symbol: PARP3
• NCBI Official Synonym Symbols: IRT1; ARTD3; ADPRT3; ADPRTL2; ADPRTL3; PADPRT-3
• NCBI Protein Information: poly [ADP-ribose] polymerase 3; poly [ADP-ribose] polymerase 3; ADPRT-3; poly[ADP-ribose] synthase 3; NAD+ ADP-ribosyltransferase 3; poly(ADP-ribose) synthetase-3; poly[ADP-ribose] synthetase 3; NAD(+) ADP-ribosyltransferase 3; ADP-ribosyltransferase diph
• UniProt Protein Name: Poly [ADP-ribose] polymerase 3
• Protein Family: Poly [ADP-ribose] polymerase
• UniProt Gene Name: PARP3
• UniProt Synonym Gene Names: ADPRT3; ADPRTL3; PARP-3; hPARP-3; ARTD3; ADPRT-3; pADPRT-3
• UniProt Entry Name: PARP3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

PARP3: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. Binds DNA. May be involved in the regulation of PRC2 and PRC3 complex-dependent gene silencing. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.2.30; DNA repair, damage; Transferase

Chromosomal Location of Human Ortholog: 3p21.2

Cellular Component: centriole; nucleus

Molecular Function: catalytic activity; NAD+ ADP-ribosyltransferase activity

Biological Process: protein amino acid ADP-ribosylation; double-strand break repair; positive regulation of DNA ligation; DNA repair; telomere maintenance