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Western Blot (WB) (Western blot analysis of PARP16 Antibody (C-term) in HepG2,MDA-MB435 cell line lysates (35ug/lane).PARP16 (arrow) was detected using the purified Pab.)

Rabbit anti-Human PARP16 Polyclonal Antibody | anti-PAR16 antibody

PARP16 Antibody (C-term)

Gene Names
PARP16; ARTD15; pART15; C15orf30
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PARP16; Polyclonal Antibody; PARP16 Antibody (C-term); Mono [ADP-ribose] polymerase PARP16; ADP-ribosyltransferase diphtheria toxin-like 15; Poly [ADP-ribose] polymerase 16; PARP-16; PAR16; anti-PAR16 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PARP16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-244 amino acids from the C-terminal region of human PARP16.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
215-244
Sequence Length
323
Applicable Applications for anti-PAR16 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of PARP16 Antibody (C-term) in HepG2,MDA-MB435 cell line lysates (35ug/lane).PARP16 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of PARP16 Antibody (C-term) in HepG2,MDA-MB435 cell line lysates (35ug/lane).PARP16 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(PARP16 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PARP16 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (PARP16 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PARP16 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PARP16 Antibody (C-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (PARP16 Antibody (C-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PAR16 antibody
The function of this protein has not been specifically defined.
References
Ame, J.C., et al. Bioessays 26(8):882-893(2004)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36383
NCBI Official Full Name
mono
NCBI Official Synonym Full Names
poly (ADP-ribose) polymerase family, member 16
NCBI Official Symbol
PARP16
NCBI Official Synonym Symbols
ARTD15; pART15; C15orf30
NCBI Protein Information
mono [ADP-ribose] polymerase PARP16
UniProt Protein Name
Mono [ADP-ribose] polymerase PARP16
UniProt Gene Name
PARP16
UniProt Synonym Gene Names
PARP-16
UniProt Entry Name
PAR16_HUMAN

Uniprot Description

PARP16: Mono-ADP-ribosyltransferase targeting the karyopherin KPNB1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transferase; EC 2.4.2.31

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane; nuclear envelope

Molecular Function: protein serine/threonine kinase activator activity; protein binding; kinase binding; NAD+ ADP-ribosyltransferase activity

Biological Process: protein amino acid ADP-ribosylation; unfolded protein response, activation of signaling protein activity; unfolded protein response

Research Articles on PAR16

Similar Products

Product Notes

The PAR16 parp16 (Catalog #AAA9203362) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 215-244. The PARP16 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARP16 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the PAR16 parp16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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