Rabbit anti-Human, mouse PARK8 (LRRK2) Polyclonal Antibody | anti-LRRK2 antibody

PARK8 (LRRK2) Antibody (L955)

Cat. Num. AAA9205211

• Gene Names: LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunofluorescence
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PARK8 (LRRK2); Polyclonal Antibody; PARK8 (LRRK2) Antibody (L955); Leucine-rich repeat serine/threonine-protein kinase 2; Dardarin; LRRK2; PARK8; anti-LRRK2 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PARK8(LRRK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 930-961 amino acids from human PARK8(LRRK2).
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 930-961
• Sequence Length: 2527

• Applicable Applications for anti-LRRK2 antibody: Western Blot (WB), ELISA (EIA), Immunofluorescence (IF)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Park8 (LRRK2) Antibody (L955) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the Park8 (LRRK2) antibody detected the Park8 (LRRK2) protein (arrow).)

Western Blot (WB)

(PARK8 (LRRK2) Antibody (L955) detect over-expressed human LRRK2 protein.)

Immunofluorescence (IF)

(Tau-stable SY5Y cell image stained for endogenous LRRK (green) byMyBioSource L955a (affinity purified), phosphorylated tau (red, monoclonalAT8) and nuclear staining by DAPI.)

Related Product Information for anti-LRRK2 antibody

Parkinson is the second most common neurodegenerative disease after Alzheimers. About 1 percent of people over the age of 65 and 3 percent of people over the age of 75 are affected by the disease. The mutation is the most common cause of Parkinson's disease identified to date. LRRK2, a genetic mutation, was recently found linked to about 5 percent of inherited cases of Parkinson's disease. By high-resolution recombination mapping and candidate gene sequencing in 46 families, 6 disease-segregating mutations (5 missense and 1 putative splice site mutation). It may be central to the pathogenesis of several major neurodegenerative disorders associated with parkinsonism. LRRK2 belongs to the ROCO protein family and includes a protein kinase domain of the MAPKKK class and several other major functional domains.

Product Categories/Family for anti-LRRK2 antibody

Neuroscience

References

Shen J. Neuron. 2004. 44:575.
Paisan-Ruiz C, et al. Neuron. 2004. 44(4):595.
Zimprich A, et al. Neuron. 2004. 44(4):601.
Wszolek ZK, et al. Neurology. 2004. 62(9):1619.
Park YJ, et al. Transplant Proc. 2004. 36(2):353.
Zimprich A, et al. Am J Hum Genet. 2004. 74:11.
Bonifati V. Lancet Neurol. 2002. 1:83.
Funayama M, et al.Ann Neurol. 2002. 51:296.

NCBI and Uniprot Product Information

• NCBI GI #: 171846278
• NCBI GeneID: 120892
• NCBI Accession #: NP_940980.3
• NCBI GenBank Nucleotide #: NM_198578.3
• UniProt Accession #: Q5S007; Q6ZS50; Q8NCX9; A6NJU2
• Molecular Weight: 286103
• NCBI Official Full Name: leucine-rich repeat serine/threonine-protein kinase 2
• NCBI Official Synonym Full Names: leucine-rich repeat kinase 2
• NCBI Official Symbol: LRRK2
• NCBI Official Synonym Symbols: PARK8; RIPK7; ROCO2; AURA17; DARDARIN
• NCBI Protein Information: leucine-rich repeat serine/threonine-protein kinase 2
• UniProt Protein Name: Leucine-rich repeat serine/threonine-protein kinase 2
• Protein Family: Leucine-rich repeat serine/threonine-protein kinase
• UniProt Gene Name: LRRK2
• UniProt Synonym Gene Names: PARK8
• UniProt Entry Name: LRRK2_HUMAN

NCBI Description

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008]

Uniprot Description

LRRK2: a large multidomain protein kinase with a TKL-type kinase domain, multiple protein-protein interaction domains and a mitochondrial Rho domain (MIRO). May play a role in the etiology of Parkinson disease. May also have GTPase activity. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Interacts with PARK2, PRDX3 and TPCN2. Expressed throughout the adult brain, but at a lower level than in heart and liver. Expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. Defects in LRRK2 are the cause of Parkinson disease type 8 (PARK8). A slowly progressive neurodegenerative disorder characterized by bradykinesia, rigidity, resting tremor, postural instability, neuronal loss in the substantia nigra, and the presence of neurofibrillary MAPT (tau)-positive and Lewy bodies in some patients.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; LRRK family

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: dendrite cytoplasm; Golgi apparatus; extracellular space; neuron projection; mitochondrion; synaptic vesicle membrane; endoplasmic reticulum; lysosome; dendrite; terminal button; perikaryon; trans-Golgi network; inclusion body; cytosol; lipid raft; mitochondrial outer membrane; cell soma; axon; mitochondrial matrix; mitochondrial inner membrane; mitochondrial membrane; cytoplasm; plasma membrane; cytoplasmic vesicle; cell junction; endosome

Molecular Function: tubulin binding; GTPase activity; identical protein binding; clathrin binding; GTP-dependent protein kinase activity; protein homodimerization activity; Rho GTPase binding; GTP binding; actin binding; GTPase activator activity; protein kinase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; SNARE binding; protein binding; protein kinase A binding; syntaxin-1 binding; kinase activity; glycoprotein binding; ATP binding

Biological Process: regulation of synaptic transmission, glutamatergic; activation of MAPKK activity; positive regulation of protein binding; activation of MAPK activity; protein amino acid autophosphorylation; regulation of neuron maturation; determination of adult life span; protein amino acid phosphorylation; positive regulation of MAP kinase activity; tangential migration from the subventricular zone to the olfactory bulb; negative regulation of protein amino acid phosphorylation; small GTPase mediated signal transduction; regulation of dopamine receptor signaling pathway; negative regulation of protein binding; regulation of mitochondrial depolarization; neuromuscular junction development; positive regulation of autophagy; positive regulation of dopamine receptor signaling pathway; olfactory bulb development; MAPKKK cascade; peptidyl-threonine phosphorylation; endocytosis; regulation of locomotion; peptidyl-serine phosphorylation; positive regulation of programmed cell death; regulation of membrane potential; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; intracellular distribution of mitochondria; autophagy; response to oxidative stress; positive regulation of protein amino acid phosphorylation; regulation of excitatory postsynaptic membrane potential; neurite morphogenesis; positive regulation of GTPase activity

Disease: Parkinson Disease 8, Autosomal Dominant