Rabbit PARK7/DJ1 Polyclonal Antibody | anti-PARK7/DJ1 antibody

PARK7/DJ1 Rabbit pAb

Cat. Num. AAA4757837

• Gene Names: PARK7; DJ1; DJ-1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: PARK7/DJ1; Polyclonal Antibody; PARK7/DJ1 Rabbit pAb; parkinson protein 7; DJ1; DJ-1; HEL-S-67p; anti-PARK7/DJ1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain (at protein level). Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa. Expressed by pancreatic islets at higher levels than surrounding exocrine tissues (PubMed:22611253).
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-PARK7/DJ1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000; ELISA: 1:1000-1:2000; IHC: 1:50-1:200
• Immunogen: Full length fusion protein
• Conjugation: Unconjugated
• Molecular Function: Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals (PubMed:25416785, PubMed:28596309). Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage (PubMed:25416785, PubMed:28013050, PubMed:26995087). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair (PubMed:28596309). Also displays an apparent glyoxalase activity that in fact reflects its deglycase activity (PubMed:22523093). Plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease; functions probably related to its primary function (PubMed:17015834, PubMed:20304780, PubMed:18711745, PubMed:12796482, PubMed:19229105, PubMed:25416785, PubMed:26995087). It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway (PubMed:12612053, PubMed:15502874, PubMed:14749723, PubMed:17015834, PubMed:21097510, PubMed:18711745). Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death (PubMed:16390825). Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria (PubMed:19229105, PubMed:16632486). Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking (PubMed:18711745). Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells (PubMed:23847046). In pancreatic islets, involved in the maintenance of mitochondrial reactive oxygen species (ROS) levels and glucose homeostasis in an age- and diet dependent manner. Protects pancreatic beta cells from cell death induced by inflammatory and cytotoxic setting. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress (PubMed:18626009). Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity (PubMed:23792957). In macrophages, interacts with the NADPH oxidase subunit NCF1 to direct NADPH oxidase-dependent ROS production, and protects against sepsis.
• Modification: Unmodified
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hela and Jurkat cell, Primary antibody: 821103(PARK7 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 821103(PARK7 Antibody) at dilution 1/25. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 821103(PARK7 Antibody) at dilution 1/25. (Original magnification: ×200))

Related Product Information for anti-PARK7/DJ1 antibody

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.

Product Categories/Family for anti-PARK7/DJ1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 183227678
• NCBI GeneID: 11315
• NCBI Accession #: NP_001116849.1
• NCBI GenBank Nucleotide #: NM_001123377.1
• UniProt Accession #: Q99497; O14805; Q6DR95; Q7LFU2; B2R4Z1
• Molecular Weight: 19,891 Da
• NCBI Official Full Name: protein DJ-1
• NCBI Official Synonym Full Names: parkinson protein 7
• NCBI Official Symbol: PARK7
• NCBI Official Synonym Symbols: DJ1; DJ-1
• NCBI Protein Information: protein DJ-1; oncogene DJ1; Parkinson disease (autosomal recessive, early onset) 7
• UniProt Protein Name: Protein DJ-1
• UniProt Gene Name: PARK7
• UniProt Entry Name: PARK7_HUMAN

NCBI Description

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.

Protein type: Nuclear receptor co-regulator; EC 3.4.-.-; Tumor suppressor; Transcription regulation

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: PML body; mitochondrion; axon; mitochondrial matrix; cytoplasm; plasma membrane; mitochondrial intermembrane space; nucleus; chromatin; cytosol; mitochondrial respiratory chain complex I; lipid raft

Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; transcription coactivator activity; mercury ion binding; transcription factor binding; peptidase activity; mRNA binding; protein binding; copper ion binding; enzyme binding; androgen receptor binding; peroxiredoxin activity; cytokine binding; double-stranded DNA binding; glyoxalase III activity; superoxide dismutase copper chaperone activity; kinase binding; single-stranded DNA binding; receptor binding

Biological Process: negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; negative regulation of protein amino acid phosphorylation; regulation of mitochondrial membrane potential; single fertilization; regulation of neuron apoptosis; enzyme active site formation via L-cysteine sulfinic acid; dopamine uptake; negative regulation of neuron apoptosis; inflammatory response; negative regulation of protein binding; mitochondrion organization and biogenesis; protein stabilization; activation of protein kinase B; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; negative regulation of protein sumoylation; positive regulation of peptidyl-serine phosphorylation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; methylglyoxal catabolic process to D-lactate; membrane depolarization; positive regulation of protein kinase B signaling cascade; detoxification of mercury ion; regulation of inflammatory response; Ras protein signal transduction; negative regulation of ubiquitin-protein ligase activity; hydrogen peroxide metabolic process; lactate biosynthetic process; autophagy; negative regulation of protein kinase activity; positive regulation of transcription factor activity; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; negative regulation of apoptosis

Disease: Parkinson Disease 7, Autosomal Recessive Early-onset