Western Blot (WB)
(PARG Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PARG antibody detected the PARG protein (arrow).)
Rabbit anti-Human PARG Polyclonal Antibody | anti-PARG antibody
PARG Antibody (C-term)
Gene Names
PARG; PARG99
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PARG; Polyclonal Antibody; PARG Antibody (C-term); Poly(ADP-ribose) glycohydrolase; anti-PARG antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PARG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 821-849 amino acids from the C-terminal region of human PARG.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
821-849
Sequence Length
976
Applicable Applications for anti-PARG antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Cellular Location
[Isoform 1]: Nucleus Note=Colocalizes withPCNA at replication foci (PubMed:21398629) Relocalizes to the cytoplasm in response toDNA damage (PubMed:16460818). [Isoform3]: Cytoplasm [Isoform 5]: Mitochondrionmatrix
Tissue Location
Ubiquitously expressed.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(PARG Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PARG antibody detected the PARG protein (arrow).)
Western Blot (WB)
(PARG Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PARG antibody detected the PARG protein (arrow).)
Related Product Information for anti-PARG antibody
Background:
Poly(ADP-ribose) glycohydrolase (PARG) is themajorenzyme responsible for the catabolism ofpoly(ADP-ribose), areversible covalent-modifier of chromosomalproteins. The proteinis found in many tissues and may be subject toproteolysisgenerating smaller, active products.Function:
Poly(ADP-ribose) glycohydrolase thatdegrades poly(ADP- ribose) by hydrolyzingthe ribose-ribose bonds present inpoly(ADP- ribose) (PubMed: 21892188,PubMed: 23102699,PubMed: 23474714). PARGacts both as an endo- and exoglycosidase,releasing poly(ADP-ribose) of differentlength as well as ADP-ribose monomers(PubMed: 23102699,PubMed: 23481255). It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated (PubMed: 21892188,PubMed: 23474714). Poly(ADP-ribose) is synthesized after DNAdamage is only present transiently and israpidly degraded by PARG (PubMed: 23102699). Required to prevent detrimentalaccumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress (PubMed: 24906880). Required for retinoid acid-dependent genetransactivation, probably by removing poly(ADP- ribose) from histone demethylase KDM4D, allowing chromatin derepression at RAR-dependent gene promoters(PubMed: 23102699). Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5(PubMed: 27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that areenergy-consuming (PubMed: 27257257).
Poly(ADP-ribose) glycohydrolase (PARG) is themajorenzyme responsible for the catabolism ofpoly(ADP-ribose), areversible covalent-modifier of chromosomalproteins. The proteinis found in many tissues and may be subject toproteolysisgenerating smaller, active products.
Poly(ADP-ribose) glycohydrolase thatdegrades poly(ADP- ribose) by hydrolyzingthe ribose-ribose bonds present inpoly(ADP- ribose) (PubMed: 21892188,PubMed: 23102699,PubMed: 23474714). PARGacts both as an endo- and exoglycosidase,releasing poly(ADP-ribose) of differentlength as well as ADP-ribose monomers(PubMed: 23102699,PubMed: 23481255). It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated (PubMed: 21892188,PubMed: 23474714). Poly(ADP-ribose) is synthesized after DNAdamage is only present transiently and israpidly degraded by PARG (PubMed: 23102699). Required to prevent detrimentalaccumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress (PubMed: 24906880). Required for retinoid acid-dependent genetransactivation, probably by removing poly(ADP- ribose) from histone demethylase KDM4D, allowing chromatin derepression at RAR-dependent gene promoters(PubMed: 23102699). Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5(PubMed: 27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that areenergy-consuming (PubMed: 27257257).
References
Whatcott, C.J., et al. Exp. Cell Res. 315(20):3477-3485(2009)
Frizzell, K.M., et al. J. Biol. Chem. 284(49):33926-33938(2009)
Erdelyi, K., et al. FASEB J. 23(10):3553-3563(2009)
Ame, J.C., et al. J. Cell. Sci. 122 (PT 12), 1990-2002 (2009) :
Uchiumi, F., et al. Genes Cells 13(12):1229-1247(2008)
Frizzell, K.M., et al. J. Biol. Chem. 284(49):33926-33938(2009)
Erdelyi, K., et al. FASEB J. 23(10):3553-3563(2009)
Ame, J.C., et al. J. Cell. Sci. 122 (PT 12), 1990-2002 (2009) :
Uchiumi, F., et al. Genes Cells 13(12):1229-1247(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
111110
NCBI Official Full Name
poly(ADP-ribose) glycohydrolase isoform a
NCBI Official Synonym Full Names
poly (ADP-ribose) glycohydrolase
NCBI Official Symbol
PARG
NCBI Official Synonym Symbols
PARG99
NCBI Protein Information
poly(ADP-ribose) glycohydrolase
UniProt Protein Name
Poly(ADP-ribose) glycohydrolase
Protein Family
UniProt Gene Name
PARG
UniProt Entry Name
PARG_HUMAN
NCBI Description
Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]
Uniprot Description
PARG: Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells. Belongs to the poly(ADP-ribose) glycohydrolase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; EC 3.2.1.143
Chromosomal Location of Human Ortholog: 10q11.23
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; mitochondrial matrix; cytoplasm; cytosol; nucleus
Molecular Function: poly(ADP-ribose) glycohydrolase activity
Biological Process: detection of bacterium; carbohydrate metabolic process; response to DNA damage stimulus
Research Articles on PARG
Similar Products
Product Notes
The PARG parg (Catalog #AAA9205623) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 821-849. The PARG Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the PARG parg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
