Goat anti-Human PARD6A Polyclonal Antibody | anti-PARD6A antibody

PARD6A, CT (Partitioning Defective 6 Homolog alpha, PAR-6, PAR-6 alpha, PAR-6A, PAR6C, Tax Interaction Protein 40, TIP-40, PAR6A)

Cat. Num. AAA6002402

• Gene Names: PARD6A; PAR6; PAR6C; TAX40; PAR-6A; TIP-40; PAR6alpha
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PARD6A; Polyclonal Antibody; CT (Partitioning Defective 6 Homolog alpha; PAR-6; PAR-6 alpha; PAR-6A; PAR6C; Tax Interaction Protein 40; TIP-40; PAR6A); Anti -PARD6A; anti-PARD6A antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human PARD6A.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-PARD6A antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:8000; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to C-GSRIRGDGSGFSL from the C-terminal of human PARD6A.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Antibody (10ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.)

Immunohistochemistry (IHC)

(Human Heart (formalin-fixed, paraffin-embedded) stained with PARD6A antibody at 5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-PARD6A antibody

PAR6 is a cell membrane protein involved in cell polarization and asymmetrical cell division processes as part of a multi protein complex. It is thought to be involved in the invasive phenotype that is associated with metastatic carcinomas, playing a role in the epithelial to mesenchymal transition (EMT). PAR6 is also thought to be involved in the formation of epithelial tight junctions. PAR6 contains a semi Cdc42/Rac interactive binding (CRIB) domain and a PDZ (PSD95/Discs-large/ZO1) domain, and several different PAR6 isoforms have been identified.

Product Categories/Family for anti-PARD6A antibody

Antibodies; Abs to Cell Division Cycle (CDC)

NCBI and Uniprot Product Information

• NCBI GI #: 82659097
• NCBI GeneID: 50855
• NCBI Accession #: NP_001032358.1
• NCBI GenBank Nucleotide #: NM_001037281.1
• UniProt Accession #: Q9NPB6; O14911; Q9NPJ7
• Molecular Weight: 37,388 Da
• NCBI Official Full Name: partitioning defective 6 homolog alpha isoform 2
• NCBI Official Synonym Full Names: par-6 family cell polarity regulator alpha
• NCBI Official Symbol: PARD6A
• NCBI Official Synonym Symbols: PAR6; PAR6C; TAX40; PAR-6A; TIP-40; PAR6alpha
• NCBI Protein Information: partitioning defective 6 homolog alpha; PAR-6 alpha; Tax-interacting protein 40; tax interaction protein 40; partitioning-defective protein 6; partitioning defective-6 homolog alpha; par-6 partitioning defective 6 homolog alpha
• UniProt Protein Name: Partitioning defective 6 homolog alpha
• Protein Family: Partitioning defective
• UniProt Gene Name: PARD6A
• UniProt Synonym Gene Names: PAR6A; PAR-6; PAR-6 alpha; PAR-6A; TIP-40
• UniProt Entry Name: PAR6A_HUMAN

NCBI Description

This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cell membrane protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. The protein also has a role in the epithelial-to-mesenchymal transition (EMT) that characterizes the invasive phenotype associated with metastatic carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

PARD6A: Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Interacts with MAP2K5. Interacts with PARD3. Interacts with GTP-bound forms of CDC42, ARHQ/TC10 and RAC1. Interacts with the N-terminal part of PRKCI and PRKCZ. Part of a complex with PARD3, CDC42 or RAC1 and PRKCI or PRKCZ. Part of a complex with LLGL1 and PRKCI. Interacts with human T-cell leukemia virus type I TAX protein. Interacts with MPP5 and CRB3. Interacts with TGFBR1; involved in TGF-beta induced epithelial to mesenchymal transition. Interacts with ECT2 ('Thr- 359' phosphorylated form) and PRKCI. Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta. Belongs to the PAR6 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: ruffle; protein complex; tight junction; apical part of cell; plasma membrane; cell cortex; nucleus; cytosol

Molecular Function: protein binding; Rho GTPase binding; protein kinase C binding; GTP-dependent protein binding; transcription factor binding

Biological Process: intercellular junction assembly and maintenance; viral reproduction; cell division; transforming growth factor beta receptor signaling pathway; intercellular junction maintenance; cell cycle