Western Blot (WB)
(Western blot analysis of PAR6G Antibody (N-term) in ZR-75-1 cell line lysates (35ug/lane). PAR6G (arrow) was detected using the purified Pab.)
Rabbit anti-Human PAR6G Polyclonal Antibody | anti-PAR6G antibody
PAR6G, NT (PARD6G, PAR6G, Partitioning defective 6 homolog gamma, PAR6D) (PE)
Gene Names
PARD6G; PAR-6G; PAR6gamma
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PAR6G; Polyclonal Antibody; NT (PARD6G; Partitioning defective 6 homolog gamma; PAR6D) (PE); PARD6G; PAR6D; anti-PAR6G antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-PAR6G antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
PAR6G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25~55 amino acids from the N-terminal region of human PAR6G.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of PAR6G Antibody (N-term) in ZR-75-1 cell line lysates (35ug/lane). PAR6G (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of PAR6G Antibody (N-term) in ZR-75-1 cell line lysates (35ug/lane). PAR6G (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(PAR6G Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PAR6G Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PAR6G Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PAR6G Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-PAR6G antibody
PAR6G involved in asymmetrical cell division and cell polarization processes. PAR6G may play a role in the formation of epithelial tight junctions. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
Product Categories/Family for anti-PAR6G antibody
References
Brajenovic, M., et al. J. Biol. Chem. 279(13):12804-12811(2004). Kohjima, M., et al. Biochem. Biophys. Res. Commun. 299(4):641-646(2002). Suzuki, A., et al. J. Cell Biol. 152(6):1183-1196(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
40,883 Da
NCBI Official Full Name
Partitioning defective 6 homolog gamma
NCBI Official Synonym Full Names
par-6 family cell polarity regulator gamma
NCBI Official Symbol
PARD6G
NCBI Official Synonym Symbols
PAR-6G; PAR6gamma
NCBI Protein Information
partitioning defective 6 homolog gamma; PAR6D; PAR-6 gamma protein; par-6 partitioning defective 6 homolog gamma
UniProt Protein Name
Partitioning defective 6 homolog gamma
UniProt Gene Name
PARD6G
UniProt Synonym Gene Names
PAR6G; PAR-6 gamma
UniProt Entry Name
PAR6G_HUMAN
Uniprot Description
PARD6G: Adapter protein involved in asymmetrical cell division and cell polarization processes. May play a role in the formation of epithelial tight junctions. The PARD6-PARD3 complex links GTP- bound Rho small GTPases to atypical protein kinase C proteins. Belongs to the PAR6 family.
Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold; Cell adhesion
Chromosomal Location of Human Ortholog: 18q23
Cellular Component: tight junction; protein complex; plasma membrane; cytosol
Molecular Function: protein binding
Biological Process: intercellular junction assembly and maintenance; cell division; cell cycle
Research Articles on PAR6G
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Product Notes
The PAR6G pard6g (Catalog #AAA6330823) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAR6G, NT (PARD6G, PAR6G, Partitioning defective 6 homolog gamma, PAR6D) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAR6G can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PAR6G pard6g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAR6G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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