Rabbit FTH1 Polyclonal Antibody | anti-FTH1 antibody

FTH1 Antibody

Cat. Num. AAA9413737

• Gene Names: FTH1; FHC; FTH; HFE5; PLIF; FTHL6; PIG15
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: FTH1; Polyclonal Antibody; FTH1 Antibody; FHC; FTH; FTHL6; MGC104426; anti-FTH1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total FTH1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 183

• Applicable Applications for anti-FTH1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human FTH1.
• Target Name: FTH1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using FTH1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat lung using FTH1 antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF-7 cell using FTH1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-FTH1 antibody

Ferritin (FTH) is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and bioavailable form (1). The assembled ferritin molecule, often referred to as a nanocage, can store up to 4,500 atoms of iron (2,3). It forms a holoenzyme of ~450 kDa, consisting of 24 subunits made up of two types of polypeptide chains: ferritin heavy chain and ferritin light chain, each having unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III) (4). In addition to iron buffering, heavy chain ferritin also enhances thymidine biosynthesis (5). Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia. The level of serum ferritin is markedly elevated in inflammation, malignancy, and iron overload disorders (6). Research studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases (7).

Product Categories/Family for anti-FTH1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 56682959
• NCBI GeneID: 2495
• NCBI Accession #: NP_002023.2
• NCBI GenBank Nucleotide #: NM_002032.2
• UniProt Accession #: P02794; Q3KRA8; Q3SWW1; B3KNR5
• Molecular Weight: 21,226 Da
• NCBI Official Full Name: ferritin heavy chain
• NCBI Official Synonym Full Names: ferritin, heavy polypeptide 1
• NCBI Official Symbol: FTH1
• NCBI Official Synonym Symbols: FHC; FTH; HFE5; PLIF; FTHL6; PIG15
• NCBI Protein Information: ferritin heavy chain
• UniProt Protein Name: Ferritin heavy chain
• Protein Family: Iron transporter
• UniProt Gene Name: FTH1
• UniProt Synonym Gene Names: FTH; FTHL6; Ferritin H subunit
• UniProt Entry Name: FRIH_HUMAN

NCBI Description

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney ().