Rabbit anti-Human, Mouse PAPOLA Polyclonal Antibody | anti-PAPOLA antibody
PAPOLA Antibody
Gene Names
PAPOLA; PAP
Reactivity
Human, Mouse
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
PAPOLA; Polyclonal Antibody; PAPOLA Antibody; MGC5378; PAP alpha; PAP; PAP-alpha; PapIII; PAPOA_HUMAN; Plap; Poly(A) polymerase alpha; Polynucleotide adenylyltransferase alpha; anti-PAPOLA antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
PAPOLA Antibody detects endogenous levels of PAPOLA.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PAPOLA antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human PAPOLA.
Conjugation
Unconjugated
Similarity
Belongs to the poly(A) polymerase family.
Post Translational Modifications
Polysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity (By similarity).Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF (By similarity).Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex (By similarity).
Subunit Structure
Monomer. Found in a complex with CPSF1, FIP1L1 and PAPOLA. Interacts with AHCYL1 and FIP1L1; the interaction with AHCYL1 seems to increase interaction with FIP1L1. Interacts with NUDT21; the interaction is diminished by acetylation. Interacts with KPNB1; the interaction promotes PAP nuclear import and is inhibited by acetylation of PAP (By similarity).
Function
Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.
Note
The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,626 Da
NCBI Official Full Name
poly(A) polymerase alpha isoform 2
NCBI Official Synonym Full Names
poly(A) polymerase alpha
NCBI Official Symbol
PAPOLA
NCBI Official Synonym Symbols
PAP
NCBI Protein Information
poly(A) polymerase alpha; poly(A) polymerase alpha; PAP-alpha; polynucleotide adenylyltransferase alpha
UniProt Protein Name
Poly(A) polymerase alpha
Protein Family
UniProt Gene Name
PAPOLA
UniProt Synonym Gene Names
PAP; PAP-alpha
UniProt Entry Name
PAPOA_HUMAN
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Product Notes
The PAPOLA papola (Catalog #AAA9630503) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAPOLA Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PAPOLA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the PAPOLA papola for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAPOLA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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