Western Blot (WB)
(PAP Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PAP antibody detected the PAP protein (arrow).)
Rabbit PAP Polyclonal Antibody | anti-PAPOLA antibody
PAP Antibody (Center)
Western Blot (WB)
(PAP Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PAP antibody detected the PAP protein (arrow).)
Western Blot (WB)
(PAP Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PAP antibody detected the PAP protein (arrow).)
Immunohistochemistry (IHC)
(PAP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PAP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Nan, H., et al. J. Invest. Dermatol. 129(9):2250-2257(2009)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Dettwiler, S., et al. J. Biol. Chem. 279(34):35788-35797(2004)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the poly(A) polymerase family. It is required for the addition of adenosine residues for the creation of the 3'-poly(A) tail of mRNAs. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
PAP-alpha: Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus. Belongs to the poly(A) polymerase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.7.19; RNA processing; Transferase; RNA splicing
Chromosomal Location of Human Ortholog: 14q32.31
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: polynucleotide adenylyltransferase activity; RNA binding; manganese ion binding; magnesium ion binding; ATP binding
Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; RNA splicing; RNA polyadenylation; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription
Research Articles on PAPOLA
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Product Notes
The PAPOLA papola (Catalog #AAA9200887) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 407-435. The PAP Antibody (Center) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's PAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the PAPOLA papola for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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