Rabbit anti-Human Pantothenate Kinase 3 Polyclonal Antibody | anti-PANK3 antibody

Pantothenate Kinase 3, NT (Pantothenic Acid Kinase 3, PANK3, hPanK3, DKFZp686O22247, FLJ12899, MGC16863) (AP)

Cat. Num. AAA6491932

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Pantothenate Kinase 3; Polyclonal Antibody; NT (Pantothenic Acid Kinase 3; PANK3; hPanK3; DKFZp686O22247; FLJ12899; MGC16863) (AP); EC=2.7.1.33; anti-PANK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PANK3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PANK3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PANK3 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PANK3 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). PANK3(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PANK3 antibody

Antibodies; Protein Kinases

References

1. Zhou, B., et al., Nat. Genet. 28(4):345-349 (2001). 2. Robishaw, J.D., et al., Am. J. Physiol. 248(1 PT 1), E1-E9 (1985).

NCBI and Uniprot Product Information

• NCBI GI #: 13375789
• NCBI GeneID: 79646
• NCBI Accession #: NP_078870
• NCBI GenBank Nucleotide #: NM_024594.3
• UniProt Accession #: Q9H999; Q53FJ9; Q7RTX4; D3DQL1
• Molecular Weight: 41,094 Da
• NCBI Official Full Name: pantothenate kinase 3
• NCBI Official Synonym Full Names: pantothenate kinase 3
• NCBI Official Symbol: PANK3
• NCBI Protein Information: pantothenate kinase 3
• UniProt Protein Name: Pantothenate kinase 3
• Protein Family: Pantothenate kinase
• UniProt Gene Name: PANK3
• UniProt Entry Name: PANK3_HUMAN

NCBI Description

This gene encodes a protein belonging to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. This family member is expressed most abundantly in the liver. [provided by RefSeq, Jul 2008]

Uniprot Description

PANK3: Plays a role in the physiological regulation of the intracellular CoA concentration. Belongs to the type II pantothenate kinase family.

Protein type: EC 2.7.1.33; Kinase, other; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: cytoplasm

Molecular Function: pantothenate kinase activity; ATP binding

Biological Process: coenzyme A biosynthetic process; phosphorylation