Rabbit PAK1 Polyclonal Antibody | anti-PAK1 antibody

PAK1 (Phospho-S199) polyclonal antibody

Cat. Num. AAA3015047

• Gene Names: PAK1; PAKalpha
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: PAK1; Polyclonal Antibody; PAK1 (Phospho-S199) polyclonal antibody; Serine/threonine-protein kinase PAK1; Alpha-PAK; p21-activated kinase 1; PAK-1; p65-PAK; anti-PAK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PAK1 (Phospho-S199) polyclonal antibody detects endogenous levels of PAK1 protein only when phosphorylated at Ser199.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PAK1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of Serine 199
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of PAK1 (Phospho-S199) polyclonal antibody at 1:500 dilutionLaneA: The Brain tissue lysate of Mouse)

Related Product Information for anti-PAK1 antibody

The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase, and growth factor-induced neurite outgrowth (1,2). Several mechanisms that induce PAK activity have been reported. Binding of Rac/Cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1). Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3). Several autophosphorylation sites have been identified, including Ser199 and Ser204 of PAK1 and Ser192 and Ser197 of PAK2 (4,5). Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6). Research indicates that phosphorylation at Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7). Phosphorylation at Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8). PAK4, PAK5, and PAK6 have lower sequence similarity with PAK1-3 in the amino-terminal regulatory region (9). Phosphorylation at Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4 (10).

NCBI and Uniprot Product Information

• NCBI GI #: 190886457
• NCBI GeneID: 5058
• NCBI Accession #: NP_001122092.1
• NCBI GenBank Nucleotide #: NM_001128620.1
• UniProt Accession #: Q13153; O75561; Q13567; Q32M53; Q32M54; Q86W79
• Molecular Weight: 60,647 Da
• NCBI Official Full Name: serine/threonine-protein kinase PAK 1 isoform 1
• NCBI Official Synonym Full Names: p21 protein (Cdc42/Rac)-activated kinase 1
• NCBI Official Symbol: PAK1
• NCBI Official Synonym Symbols: PAKalpha
• NCBI Protein Information: serine/threonine-protein kinase PAK 1; p65-PAK; alpha-PAK; STE20 homolog, yeast; p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
• UniProt Protein Name: Serine/threonine-protein kinase PAK 1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK1
• UniProt Synonym Gene Names: PAK-1
• UniProt Entry Name: PAK1_HUMAN

NCBI Description

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Uniprot Description

PAK1: a protein kinase of the STE20 family that regulates cell motility and morphology. Critical RhoGTPase effector that regulates cytoskeleton reorganization, the JNK MAPK pathway, and nuclear signaling. Binding of Rac/cdc42 to the PBD of PAK1 causes autophosphorylation and conformational change. Phosphorylated and activated by PDK.

Protein type: EC 2.7.11.1; Protein kinase, STE; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKA subfamily

Chromosomal Location of Human Ortholog: 11q13-q14

Cellular Component: Golgi apparatus; nuclear membrane; focal adhesion; protein complex; dendrite; intercellular junction; Z disc; cytosol; filamentous actin; ruffle; growth cone; axon; cytoplasm; plasma membrane

Molecular Function: collagen binding; protein serine/threonine kinase activity; protein binding; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: axon guidance; positive regulation of JNK activity; exocytosis; wound healing; apoptosis; protein amino acid autophosphorylation; positive regulation of estrogen receptor signaling pathway; receptor clustering; T cell receptor signaling pathway; protein amino acid phosphorylation; positive regulation of stress fiber formation; ephrin receptor signaling pathway; dendrite development; neuromuscular junction development; MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; cellular response to insulin stimulus; branching morphogenesis of a tube; actin cytoskeleton reorganization; T cell costimulation; response to hypoxia; innate immune response; positive regulation of protein amino acid phosphorylation; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; neurite morphogenesis