Rabbit anti-Human PAK1 Polyclonal Antibody | anti-PAK1 antibody

PAK1 (T423) (Serine/Threonine-protein Kinase PAK 1, p21-activated Kinase 1, PAK-1, p65-PAK, Alpha-PAK) (FITC)

Cat. Num. AAA6491793

• Gene Names: PAK1; IDDMSSD; p65-PAK; PAKalpha; alpha-PAK
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: PAK1; Polyclonal Antibody; PAK1 (T423) (Serine/Threonine-protein Kinase PAK 1; p21-activated Kinase 1; PAK-1; p65-PAK; Alpha-PAK) (FITC); anti-PAK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PAK1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 545

• Applicable Applications for anti-PAK1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding T423 of human PAK1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PAK1 (arrow) using rabbit polyclonal PAK1 Antibody (T423).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK1 gene (Lane 2) (Origene Technologies).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PAK1 Antibody (T423) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PAK1 Antibody (T423), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-PAK1 antibody

Antibodies; Protein Kinases

References

1. Chen, S., et al., J. Biol. Chem. 278(22):20029-20036 (2003). 2. Sells, M.A., et al., Curr. Biol. 7(3):202-210 (1997). 3. Brown, J.L., et al., Curr. Biol. 6(5):598-605 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 42794769
• NCBI GeneID: 5058
• NCBI Accession #: NP_002567
• NCBI GenBank Nucleotide #: NP_002567.3
• UniProt Accession #: Q13153
• Molecular Weight: 60.661
• NCBI Official Full Name: serine/threonine-protein kinase PAK 1 isoform 2
• NCBI Official Synonym Full Names: p21 (RAC1) activated kinase 1
• NCBI Official Symbol: PAK1
• NCBI Official Synonym Symbols: IDDMSSD; p65-PAK; PAKalpha; alpha-PAK
• NCBI Protein Information: serine/threonine-protein kinase PAK 1
• UniProt Protein Name: Serine/threonine-protein kinase PAK 1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK1
• UniProt Synonym Gene Names: PAK-1
• UniProt Entry Name: PAK1_HUMAN

NCBI Description

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Uniprot Description

PAK1: a protein kinase of the STE20 family that regulates cell motility and morphology. Critical RhoGTPase effector that regulates cytoskeleton reorganization, the JNK MAPK pathway, and nuclear signaling. Binding of Rac/cdc42 to the PBD of PAK1 causes autophosphorylation and conformational change. Phosphorylated and activated by PDK.

Protein type: EC 2.7.11.1; Protein kinase, STE; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKA subfamily

Chromosomal Location of Human Ortholog: 11q13-q14

Cellular Component: Golgi apparatus; nuclear membrane; focal adhesion; protein complex; dendrite; intercellular junction; Z disc; cytosol; filamentous actin; ruffle; growth cone; axon; cytoplasm; plasma membrane

Molecular Function: collagen binding; protein serine/threonine kinase activity; protein binding; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: axon guidance; positive regulation of JNK activity; exocytosis; wound healing; apoptosis; protein amino acid autophosphorylation; positive regulation of estrogen receptor signaling pathway; receptor clustering; T cell receptor signaling pathway; protein amino acid phosphorylation; positive regulation of stress fiber formation; ephrin receptor signaling pathway; dendrite development; neuromuscular junction development; MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; cellular response to insulin stimulus; branching morphogenesis of a tube; actin cytoskeleton reorganization; T cell costimulation; response to hypoxia; innate immune response; positive regulation of protein amino acid phosphorylation; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; neurite morphogenesis