Human Pulmonary Activation Regulated Chemokine (PARC) RTU ELISA Kit | PARC rtu elisa kit

Human Pulmonary Activation Regulated Chemokine (PARC) ELISA Kit

Cat. Num. AAA4501662

• Gene Names: CCL18; CKb7; PARC; AMAC1; DCCK1; MIP-4; AMAC-1; DC-CK1; SCYA18
• Reactivity: Human
• Synonyms: Pulmonary Activation Regulated Chemokine (PARC); Human Pulmonary Activation Regulated Chemokine (PARC) ELISA Kit; CCL18; AMAC1; SCYA18; DCCK1; MIP-4; CKb7; Alternative Macrophage Activation Associated CC Chemokine 1; Chemokine C-C-Motif Ligand 18; Macrophage inflammatory protein 4; PARC rtu elisa kit

• Reactivity: Human
• Sequence Length: 89

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 30pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PARC rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PARC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PARC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PARC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PARC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506831
• NCBI GeneID: 6362
• NCBI Accession #: NP_002979.1
• NCBI GenBank Nucleotide #: NM_002988.3
• UniProt Accession #: P55774; Q53X71; B5BUM2
• Molecular Weight: 9,849 Da
• NCBI Official Full Name: C-C motif chemokine 18
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 18
• NCBI Official Symbol: CCL18
• NCBI Official Synonym Symbols: CKb7; PARC; AMAC1; DCCK1; MIP-4; AMAC-1; DC-CK1; SCYA18
• NCBI Protein Information: C-C motif chemokine 18
• UniProt Protein Name: C-C motif chemokine 18
• Protein Family: Protein
• UniProt Gene Name: CCL18
• UniProt Synonym Gene Names: AMAC1; DCCK1; MIP4; PARC; SCYA18; AMAC-1; DC-CK1; MIP-4

NCBI Description

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for naive T cells, CD4+ and CD8+ T cells and nonactivated lymphocytes, but not for monocytes or granulocytes. This chemokine attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes. It may play a role in both humoral and cell-mediated immunity responses. [provided by RefSeq, Sep 2014]

Uniprot Description

Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.