Western Blot (WB)
(Western Blot: Sample: Rat Stomach Tissue.)
Rabbit anti-Rat Paired Box Gene 6 Polyclonal Antibody | anti-PAX6 antibody
Paired Box Gene 6 (PAX6) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Rat Stomach Tissue.)
Western Blot (WB)
(Western Blot: Sample: Rat Stomach Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PAX6, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PAX6, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
NCBI and Uniprot Product Information
NCBI Description
homeodomain (HD)-containing transcription factor; may play a role in the differentiation of the hindbrain somatic (SM) motoneurones and V1 interneurones in the hindbrain and/or spinal cord [RGD, Feb 2006]
Uniprot Description
PAX6: Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells. Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Defects in PAX6 are the cause of aniridia (AN). A congenital, bilateral, panocular disorder characterized by complete absence of the iris or extreme iris hypoplasia. Aniridia is not just an isolated defect in iris development but it is associated with macular and optic nerve hypoplasia, cataract, corneal changes, nystagmus. Visual acuity is generally low but is unrelated to the degree of iris hypoplasia. Glaucoma is a secondary problem causing additional visual loss over time. Defects in PAX6 are a cause of Peters anomaly (PAN). Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea. Defects in PAX6 are a cause of foveal hypoplasia (FOVHYP). Foveal hypoplasia can be isolated or associated with presenile cataract. Inheritance is autosomal dominant. Defects in PAX6 are a cause of keratitis hereditary (KERH). An ocular disorder characterized by corneal opacification, recurrent stromal keratitis and vascularization. Defects in PAX6 are a cause of coloboma of iris choroid and retina (COI); also known as uveoretinal coloboma. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Severe colobomatous malformations may cause as much as 10% of the childhood blindness. The clinical presentation of ocular coloboma is variable. Some individuals may present with minimal defects in the anterior iris leaf without other ocular defects. More complex malformations create a combination of iris, uveoretinal and/or optic nerve defects without or with microphthalmia or even anophthalmia. Defects in PAX6 are a cause of coloboma of optic nerve (COLON). Defects in PAX6 are a cause of bilateral optic nerve hypoplasia (BONH); also known as bilateral optic nerve aplasia. A congenital anomaly in which the optic disc appears abnormally small. It may be an isolated finding or part of a spectrum of anatomic and functional abnormalities that includes partial or complete agenesis of the septum pellucidum, other midline brain defects, cerebral anomalies, pituitary dysfunction, and structural abnormalities of the pituitary. Defects in PAX6 are a cause of aniridia cerebellar ataxia and mental deficiency (ACAMD); also known as Gillespie syndrome. A rare condition consisting of partial rudimentary iris, cerebellar impairment of the ability to perform coordinated voluntary movements, and mental retardation. Belongs to the paired homeobox family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Motility/polarity/chemotaxis; Transcription factor
Cellular Component: cytoplasm; cytosol; intracellular; nuclear chromatin; nucleoplasm; nucleus
Molecular Function: AT DNA binding; chromatin binding; DNA binding; DNA binding transcription factor activity; double-stranded DNA binding; histone acetyltransferase binding; protein kinase binding; sequence-specific DNA binding; transcription factor binding; ubiquitin protein ligase binding; ubiquitin-protein transferase activity
Biological Process: anterior/posterior pattern specification; astrocyte differentiation; axon guidance; axonogenesis; blood vessel development; brain development; camera-type eye development; cell differentiation; cell fate commitment; cell fate determination; cellular response to insulin stimulus; central nervous system development; cerebellum development; cerebral cortex regionalization; commitment of a neuronal cell to a specific type of neuron in the forebrain; dorsal/ventral axis specification; dorsal/ventral pattern formation; embryonic camera-type eye morphogenesis; epithelial cell development; establishment of mitotic spindle orientation; eye photoreceptor cell development; forebrain development; forebrain dorsal/ventral pattern formation; forebrain-midbrain boundary formation; glucose homeostasis; habenula development; hindbrain development; interkinetic nuclear migration; keratinocyte differentiation; lacrimal gland development; lens development in camera-type eye; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of neurogenesis; negative regulation of neuron differentiation; negative regulation of protein phosphorylation; negative regulation of transcription from RNA polymerase II promoter; neural crest cell migration; neuron migration; olfactory bulb development; oligodendrocyte cell fate specification; pallium development; pituitary gland development; positive regulation of cell fate specification; positive regulation of epithelial cell differentiation; positive regulation of neuroblast proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; protein localization in organelle; protein ubiquitination; regionalization; regulation of asymmetric cell division; regulation of cell migration; regulation of gene expression; regulation of neurogenesis; regulation of neuron differentiation; regulation of timing of cell differentiation; regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter involved in somatic motor neuron fate commitment; regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification; regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification; response to ethanol; response to wounding; retina development in camera-type eye; rhombomere morphogenesis; salivary gland morphogenesis; smoothened signaling pathway; spinal cord development; telencephalon regionalization; transcription from RNA polymerase II promoter
Research Articles on PAX6
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Product Notes
The PAX6 pax6 (Catalog #AAA2032746) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Paired Box Gene 6 (PAX6) Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Paired Box Gene 6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PAX6 pax6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Paired Box Gene 6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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