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Western Blot (WB) (Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 231 cells, Jurkat cells, Primary antibody: 861066(PAFAH2 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

Rabbit anti-Human PAFAH2 Polyclonal Antibody | anti-PAFAH2 antibody

PAFAH2 Rabbit pAb

Gene Names
PAFAH2; HSD-PLA2
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
PAFAH2; Polyclonal Antibody; PAFAH2 Rabbit pAb; platelet-activating factor acetylhydrolase 2; 40kDa; HSD-PLA2; anti-PAFAH2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-PAFAH2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
IHC: 1:100-1:300
Immunogen
Synthetic peptide of human PAFAH2
Conjugation
Unconjugated
Modification
Unmodified
Cell Position
Cytoplasm, Endoplasmic reticulum, Membrane
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 231 cells, Jurkat cells, Primary antibody: 861066(PAFAH2 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

Western Blot (WB) (Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 231 cells, Jurkat cells, Primary antibody: 861066(PAFAH2 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 861066(PAFAH2 Antibody) at dilution 1/80, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 861066(PAFAH2 Antibody) at dilution 1/80, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 861066(PAFAH2 Antibody) at dilution 1/80, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 861066(PAFAH2 Antibody) at dilution 1/80, on the right is treated with synthetic peptide. (Original magnification: ×200))
Product Categories/Family for anti-PAFAH2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46.4kDa (415aa) confirmed by MALDI-TOF
NCBI Official Full Name
platelet-activating factor acetylhydrolase 2, cytoplasmic
NCBI Official Synonym Full Names
platelet activating factor acetylhydrolase 2
NCBI Official Symbol
PAFAH2
NCBI Official Synonym Symbols
HSD-PLA2
NCBI Protein Information
platelet-activating factor acetylhydrolase 2, cytoplasmic
UniProt Protein Name
Platelet-activating factor acetylhydrolase 2, cytoplasmic
UniProt Gene Name
PAFAH2
UniProt Synonym Gene Names
SD-PLA2; hSD-PLA2
UniProt Entry Name
PAFA2_HUMAN

NCBI Description

This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme. [provided by RefSeq, Jul 2008]

Uniprot Description

PAFAH2: Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme. Belongs to the serine esterase family.

Protein type: EC 3.1.1.47; Hydrolase; Lipid Metabolism - ether lipid; Lipid-binding

Chromosomal Location of Human Ortholog: 1p36

Molecular Function: 1-alkyl-2-acetylglycerophosphocholine esterase activity; phospholipid binding

Biological Process: lipid metabolic process

Research Articles on PAFAH2

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Product Notes

The PAFAH2 pafah2 (Catalog #AAA4758351) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAFAH2 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAFAH2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC: 1:100-1:300. Researchers should empirically determine the suitability of the PAFAH2 pafah2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAFAH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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