Rabbit anti-Human, mouse Pael-R (GPR37) Polyclonal Antibody | anti-GPR37 antibody

Pael-R (GPR37) Antibody (N-term)

Cat. Num. AAA9204126

• Gene Names: GPR37; PAELR; EDNRBL; hET(B)R-LP
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Pael-R (GPR37); Polyclonal Antibody; Pael-R (GPR37) Antibody (N-term); Prosaposin receptor GPR37; Endothelin B receptor-like protein 1; ETBR-LP-1; G-protein coupled receptor 37; Parkin-associated endothelin receptor-like receptor; PAELR; GPR37; anti-GPR37 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Pael-R (GPR37) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-126 amino acids from the N-terminal region of human Pael-R (GPR37).
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 97-126
• Sequence Length: 613

• Applicable Applications for anti-GPR37 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-Pael-R (GPR37) Pab in mouse brain tissue lysate. Pael-R (GPR37) (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Pael Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Pael antibody detected the Pael protein (arrow).)

Related Product Information for anti-GPR37 antibody

Parkinson is the second most common neurodegenerative disease after Alzheimers. About 1 percent of people over the age of 65 and 3 percent of people over the age of 75 are affected by the disease. The mutation is the most common cause of Parkinson disease identified to date. The function of Park2 is not well-known; however, it may play a role in the ubiquitin-mediated proteolytic pathway. Mutations in this gene are known to cause autosomal recessive juvenile parkinsonism. Alternative splicing of this gene produces three known products of undetermined function. Panneuronal expression of Parkin substrate Pael-R causes age-dependent selective degeneration of Drosophila dopaminergic (DA) neurons; coexpression of Parkin degrades Pael-R and suppresses its toxicity.

Product Categories/Family for anti-GPR37 antibody

Signal Transduction; GPCR Antibodies

References

Yang,Y., et al. Neuron 37 (6), 911-924 (2003) Imai,Y., et al. Mol. Cell 10 (1), 55-67 (2002) Imai,Y., et al. Cell 105 (7), 891-902 (2001) Marazziti,D., et al. Genomics 45 (1), 68-77 (1997) Zeng,Z., et al. BBRC 233 (2), 559-567 (1997)

NCBI and Uniprot Product Information

• NCBI GI #: 4885323
• NCBI GeneID: 2861
• NCBI Accession #: NP_005293.1
• NCBI GenBank Nucleotide #: NM_005302.3
• UniProt Accession #: O15354; O00348; O14768; Q8TD39; A4D0Y6
• Molecular Weight: 67114
• NCBI Official Full Name: prosaposin receptor GPR37
• NCBI Official Synonym Full Names: G protein-coupled receptor 37 (endothelin receptor type B-like)
• NCBI Official Symbol: GPR37
• NCBI Official Synonym Symbols: PAELR; EDNRBL; hET(B)R-LP
• NCBI Protein Information: prosaposin receptor GPR37
• UniProt Protein Name: Prosaposin receptor GPR37
• Protein Family: Prosaposin receptor
• UniProt Gene Name: GPR37
• UniProt Synonym Gene Names: ETBR-LP-1; PAELR
• UniProt Entry Name: GPR37_HUMAN

NCBI Description

This gene is a member of the G protein-coupled receptor family. The encoded protein contains seven transmembrane domains and is found in cell and endoplasmic reticulum membranes. G protein-coupled receptors are involved in translating outside signals into G protein mediated intracellular effects. This gene product interacts with Parkin and is involved in juvenile Parkinson disease. [provided by RefSeq, Oct 2012]

Uniprot Description

GPR37: Orphan receptor. May have a unique functional role in the central nervous system. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane; receptor complex; ubiquitin ligase complex

Molecular Function: G-protein coupled receptor activity; protein binding; ubiquitin protein ligase binding; heat shock protein binding; peptide receptor activity, G-protein coupled; Hsp70 protein binding; peptide binding

Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of dopamine metabolic process; positive regulation of MAPKKK cascade; G-protein signaling, adenylate cyclase inhibiting pathway; dopamine biosynthetic process; locomotion during locomotory behavior