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Western Blot (WB) (Western Blotting Analysis: Representative lot data. Mouse brain tissue lysate was probed with Anti-Endophilin-A1 (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and detected using a chemiluminescence detection system. Arrow indicates Endophilin-A1 (~42kD). Note: Film exposure time: 15 min.)

Rabbit anti-Mouse, Rat PACSIN3 Polyclonal Antibody | anti-PACSIN3 antibody

PACSIN3 (Protein Kinase C and Casein Kinase Substrate in Neurons 3, PACSIN-3, Endophilin 1, Endophilin I, Endophilin-I, SH3 Domain-containing Protein 6511)

Gene Names
PACSIN3; SDPIII; FLJ17861
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
PACSIN3; Polyclonal Antibody; PACSIN3 (Protein Kinase C and Casein Kinase Substrate in Neurons 3; PACSIN-3; Endophilin 1; Endophilin I; Endophilin-I; SH3 Domain-containing Protein 6511); Anti -PACSIN3 (Protein Kinase C and Casein Kinase Substrate in Neurons 3; anti-PACSIN3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Endophilin-A1. Species Crossreactivity: rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.05% sodium azide, 50% glycerol.
Applicable Applications for anti-PACSIN3 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:500
Immunogen
Linear peptide corresponding to mouse Endophilin-A1(KLH).
Positive Control
Mouse brain tissue lysate.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blotting Analysis: Representative lot data. Mouse brain tissue lysate was probed with Anti-Endophilin-A1 (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and detected using a chemiluminescence detection system. Arrow indicates Endophilin-A1 (~42kD). Note: Film exposure time: 15 min.)

Western Blot (WB) (Western Blotting Analysis: Representative lot data. Mouse brain tissue lysate was probed with Anti-Endophilin-A1 (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and detected using a chemiluminescence detection system. Arrow indicates Endophilin-A1 (~42kD). Note: Film exposure time: 15 min.)

Western Blot (WB)

(Western Blotting Analysis Representative lot data: Rat brain (lane 1) and mouse brain (lane 2) membrane tissue lysates were probed with Anti-Endophilin-A1 (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and detected using a chemiluminescence detection system. Arrow indicates Endophilin-A1 (~42kD). Note: Film exposure time: 15 min.)

Western Blot (WB) (Western Blotting Analysis Representative lot data: Rat brain (lane 1) and mouse brain (lane 2) membrane tissue lysates were probed with Anti-Endophilin-A1 (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and detected using a chemiluminescence detection system. Arrow indicates Endophilin-A1 (~42kD). Note: Film exposure time: 15 min.)
Related Product Information for anti-PACSIN3 antibody
A member of the Endophilin family, Endophilin A1 is a protein containing SH3 domains, and has been implicated in the trafficking of clathrin-mediated synaptic vesicle endocytosis within the membrane. It was originally characterized as a presynaptically enriched protein that binds to endocytic proteins polyphosphoinositide synaptojanin and dynamin. Endophilin A1 has been implicated in endocytic vesicle budding when its function in cell-free systems and in living synapses has been disturbed. Along with the SH3 domain, Endophilin A1 contains a BAR domain in the N-terminal region, that has been found to induce and bind membrane curvature. This protein's expression is localized to the brain, most specifically in the frontal cortex. Markedly high expression is observed in fetal cerebellum.
Product Categories/Family for anti-PACSIN3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,487 Da
NCBI Official Full Name
protein kinase C and casein kinase substrate in neurons protein 3
NCBI Official Synonym Full Names
protein kinase C and casein kinase substrate in neurons 3
NCBI Official Symbol
PACSIN3
NCBI Official Synonym Symbols
SDPIII; FLJ17861
NCBI Protein Information
protein kinase C and casein kinase substrate in neurons protein 3; syndapin III; OTTHUMP00000234262; OTTHUMP00000234263; OTTHUMP00000234264; SH3 domain-containing protein 6511
UniProt Protein Name
Protein kinase C and casein kinase substrate in neurons protein 3
UniProt Gene Name
PACSIN3
UniProt Entry Name
PACN3_HUMAN

NCBI Description

This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

PACSIN3: May play a role in vesicle formation and transport. Belongs to the PACSIN family.

Protein type: Adaptor/scaffold; Vesicle

Chromosomal Location of Human Ortholog: 11p12-p11.12

Cellular Component: nucleoplasm; membrane; cytoplasm; plasma membrane; endosome

Molecular Function: protein binding; cytoskeletal protein binding; phospholipid binding; calcium channel inhibitor activity; lipid binding

Biological Process: negative regulation of calcium ion transport; negative regulation of endocytosis; regulation of endocytosis; endocytosis; cytoskeleton organization and biogenesis; positive regulation of membrane protein ectodomain proteolysis

Research Articles on PACSIN3

Similar Products

Product Notes

The PACSIN3 pacsin3 (Catalog #AAA625347) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PACSIN3 (Protein Kinase C and Casein Kinase Substrate in Neurons 3, PACSIN-3, Endophilin 1, Endophilin I, Endophilin-I, SH3 Domain-containing Protein 6511) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PACSIN3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:500. Researchers should empirically determine the suitability of the PACSIN3 pacsin3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PACSIN3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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