Rabbit anti-Human, Mouse PACSIN3 Polyclonal Antibody | anti-PACSIN3 antibody

PACSIN3, NT (Protein Kinase C And Casein Kinase Substrate In Neurons Protein 3, SH3 Domain-containing Protein 6511, Endophilin I) (Azide free) (HRP)

Cat. Num. AAA6491695

• Gene Names: PACSIN3; SDPIII
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PACSIN3; Polyclonal Antibody; NT (Protein Kinase C And Casein Kinase Substrate In Neurons Protein 3; SH3 Domain-containing Protein 6511; Endophilin I) (Azide free) (HRP); anti-PACSIN3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PACSIN3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PACSIN3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PACSIN3 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PACSIN3 Pab is used in Western blot to detect PACSIN3 in mouse brain tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(PACSIN3 Antibody (M1) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PACSIN3 antibody detected the PACSIN3 protein (arrow).)

Western Blot (WB)

(Western blot analysis of PACSIN3 (arrow) using rabbit polyclonal PACSIN3 Antibody (M1). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PACSIN3 gene.)

Product Categories/Family for anti-PACSIN3 antibody

Antibodies; Protein Kinases

References

1. Howard, L., et al., J. Biol. Chem. 274(44):31693-31699 (1999). 2. Modregger, J., et al., J. Cell. Sci. 113 Pt 24, 4511-4521 (2000). 3. Sumoy, L., et al., Gene 262(1-2), 199-205 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 296923797
• NCBI GeneID: 29763
• NCBI Accession #: NP_001171903
• NCBI GenBank Nucleotide #: NM_001184974
• UniProt Accession #: Q9UKS6; Q9H331; Q9NWV9; A6NH84
• Molecular Weight: 48,487 Da
• NCBI Official Full Name: protein kinase C and casein kinase substrate in neurons protein 3
• NCBI Official Synonym Full Names: protein kinase C and casein kinase substrate in neurons 3
• NCBI Official Symbol: PACSIN3
• NCBI Official Synonym Symbols: SDPIII
• NCBI Protein Information: protein kinase C and casein kinase substrate in neurons protein 3; SH3 domain-containing protein 6511; syndapin III
• UniProt Protein Name: Protein kinase C and casein kinase substrate in neurons protein 3
• Protein Family: Protein kinase C and casein kinase II substrate protein
• UniProt Gene Name: PACSIN3
• UniProt Entry Name: PACN3_HUMAN