Rabbit PACSIN1 Polyclonal Antibody | anti-PACSIN1 antibody

PACSIN1 Antibody

Cat. Num. AAA9630554

• Gene Names: PACSIN1; SDPI
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PACSIN1; Polyclonal Antibody; PACSIN1 Antibody; A830061D09Rik; Dynamin PRD-interacting protein; Dynamin proline-rich domain-interacting protein; H74; KIAA1379; MGC114042; MGC56324; mKIAA1379; OTTHUMP00000016231; PACN1_HUMAN; Pacsin1; PASCIN; Protein kinase C and casein kinase substrate in neurons 1; Protein kinase C and casein kinase substrate in neurons protein 1; SDPI; Synaptic; dynamin-associated protein I; Syndapin 1; Syndapin; Syndapin-I; zgc:114042; zgc:56324; anti-PACSIN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PACSIN1 Antibody detects endogenous levels of PACSIN1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PACSIN1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human PACSIN1.
• Conjugation: Unconjugated
• Similarity: The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain. DNM1 binding abolishes autoinhibition (By similarity). Belongs to the PACSIN family.
• Post Translational Modifications: Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
• Subunit Structure: May form heterooligomers with other PACSINs. Interacts with MAPT. Interacts with TRPV4 (By similarity). Interacts (via SH3 domain) with SYNJ1 and WASL. Interacts with DNM2 and DNM3. Interacts with both COBL and DBNL. Identified in a complex composed of COBL, PACSIN1 and WASL. Interacts with EHD1 and EHD3 (By similarity). Homodimer. Interacts (via SH3 domain) with DNM1; the interaction is reduced by DNM1 phosphorylation.
• Tissue Specificity: Highly expressed in brain and, at much lower levels, in heart and pancreas.
• Function: Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission (By similarity). Binds to membranes via its F-BAR domain and mediates membrane tubulation.
• Note: Colocalizes with MAPT in axons. In primary neuronal cultures, present at a high level in presynaptic nerve terminals and in the cell body. Colocalizes with DNM1 at vesicular structures in the cell body and neurites (By similarity). Associates with membranes via its F-BAR domain.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PACSIN1 antibody

Cytoplasm; Cell projection; Cell junction; Synapse; Synaptosome; Ruffle membrane; Membrane; Peripheral membrane protein; Cytoplasmic vesicle membrane; Cytosol; Cell membrane; Cytoplasmic side; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 313760653
• NCBI GeneID: 29993
• NCBI Accession #: NP_001186512.1
• NCBI GenBank Nucleotide #: NM_001199583.2
• UniProt Accession #: Q9BY11; Q9P2G8
• Molecular Weight: 51kDa
• NCBI Official Full Name: protein kinase C and casein kinase substrate in neurons protein 1
• NCBI Official Synonym Full Names: protein kinase C and casein kinase substrate in neurons 1
• NCBI Official Symbol: PACSIN1
• NCBI Official Synonym Symbols: SDPI
• NCBI Protein Information: protein kinase C and casein kinase substrate in neurons protein 1; syndapin 1; syndapin I
• UniProt Protein Name: Protein kinase C and casein kinase substrate in neurons protein 1
• Protein Family: Protein kinase C and casein kinase substrate in neurons protein
• UniProt Gene Name: PACSIN1
• UniProt Synonym Gene Names: KIAA1379
• UniProt Entry Name: PACN1_HUMAN