Rabbit anti-Human PAAF1 Polyclonal Antibody | anti-PAAF1 antibody

PAAF1, NT (PAAF1, WDR71, Proteasomal ATPase-associated factor 1, Protein G-16, WD repeat-containing protein 71) (APC)

Cat. Num. AAA6330341

• Gene Names: PAAF1; PAAF; Rpn14; WDR71
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PAAF1; Polyclonal Antibody; NT (PAAF1; WDR71; Proteasomal ATPase-associated factor 1; Protein G-16; WD repeat-containing protein 71) (APC); WD repeat-containing protein 71; anti-PAAF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PAAF1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PAAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-145 amino acids from the N-terminal region of human PAAF1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PAAF1 Antibody (N-term) in A549 cell line lysates (35ug/lane).PAAF1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(PAAF1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PAAF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PAAF1 antibody

PAAF1 inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S regulatory complex through dissociation of the proteasome 26S. This presumably promotes provirus transcription efficiency.

Product Categories/Family for anti-PAAF1 antibody

Antibodies; WD Repeat Proteins

References

Lassot, I., et al. Mol. Cell 25(3):369-383(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007). Ponnappan, S., et al. Int. J. Biochem. Cell Biol. 39(4):799-809(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 392513656
• NCBI GeneID: 80227
• NCBI Accession #: NP_001254734.1
• NCBI GenBank Nucleotide #: NM_001267805.1
• UniProt Accession #: Q9BRP4; Q4G165; Q53HS9; Q7Z500; Q8TBU6; Q9HAB6; A6NDR5; B4DPB0; B7ZAS9
• Molecular Weight: 29,982 Da
• NCBI Official Full Name: proteasomal ATPase-associated factor 1 isoform 1
• NCBI Official Synonym Full Names: proteasomal ATPase associated factor 1
• NCBI Official Symbol: PAAF1
• NCBI Official Synonym Symbols: PAAF; Rpn14; WDR71
• NCBI Protein Information: proteasomal ATPase-associated factor 1
• UniProt Protein Name: Proteasomal ATPase-associated factor 1
• Protein Family: Proteasomal ATPase-associated factor
• UniProt Gene Name: PAAF1
• UniProt Synonym Gene Names: WDR71

NCBI Description

This gene encodes a WD repeat-containing protein involved in regulation of association of proteasome components. During HIV infection, the encoded protein is thought to promote provirus transcription through recruitment of the 19S regulatory complex. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2012]

Uniprot Description

Inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S regulatory complex through dissociation of the proteasome 26S. This presumably promotes provirus transcription efficiency. Protects SUPT6H from proteasomal degradation.