Western Blot (WB)
(Western blot analysis of Mouse brain cell lysates showing detection of p70 S6K protein using Rabbit Anti-p70 S6K Polyclonal Antibody (SPC-146). Primary Antibody: Rabbit Anti-p70 S6K Polyclonal Antibody (SPC-146) at 1:1000. Anti-p70 S6K Antibody on right, IP negative control on the left.)
Rabbit p70 S6K Polyclonal Antibody | anti-RPS6KB2 antibody
p70 S6K Antibody: HRP
Gene Names
RPS6KB2; KLS; SRK; S6K2; STK14B; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta
Reactivity
Human, Mouse, Rat, Bovine
Applications
Western Blot, Immunoprecipitation, ELISA
Synonyms
p70 S6K; Polyclonal Antibody; p70 S6K Antibody: HRP; p70 S6 kinase beta (phospho T229); p70-beta; p70S6Kb; S6K beta; S6K2; SRK; STK14B; anti-RPS6KB2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine
Clonality
Polyclonal
Form/Format
Affinity Purified
Concentration
250ug/mL (varies by lot)
Sequence Length
482
Applicable Applications for anti-RPS6KB2 antibody
Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
Application Notes
1ug/mL (WB)
Conjugate
HRP
Storage Buffer
TBS, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western blot analysis of Mouse brain cell lysates showing detection of p70 S6K protein using Rabbit Anti-p70 S6K Polyclonal Antibody (SPC-146). Primary Antibody: Rabbit Anti-p70 S6K Polyclonal Antibody (SPC-146) at 1:1000. Anti-p70 S6K Antibody on right, IP negative control on the left.)
Western Blot (WB)
(Western blot analysis of Mouse brain cell lysates showing detection of p70 S6K protein using Rabbit Anti-p70 S6K Polyclonal Antibody (SPC-146). Primary Antibody: Rabbit Anti-p70 S6K Polyclonal Antibody (SPC-146) at 1:1000. Anti-p70 S6K Antibody on right, IP negative control on the left.)
Related Product Information for anti-RPS6KB2 antibody
Background Info: Detects a ~70kDa protein corresponding to the molecular mass of p70 S6K on SDS PAGE immunoblots.
Scientific Background: Ribosomal S6 (S6K) belongs to the AGC family of Serine/Threonine protein kinases. There are two forms of S6K, alpha and beta, which have cytoplasmic and nuclear variants derived from alternative splicing (1). The activity of S6K is regulated by phosphorylation/ dephosphorylation events in cellular responses to various extracellular stimuli. Its activity has also been shown to be dependent on nutrient availability (3-6). The mechanism of activation of S6K is a multi-step process which is achieved by phosphorylating the 40S ribosomal protein S6, which then upregulates the translation of mRNA transcripts containing an oligopryimidine tract at the 5' transcriptional start site (3-6). Recently, a highly homologous ribosomal S6 kinase, termed S6K2, was identified. It has 70% amino acid identity in the overall sequence with S6K1, and highly conserved potential phosphorylation sites of S6K1. However, the N- and C-terminal domains of S6K2 are quite different (2). As a result, S6K is a key player in the regulation of cell size, growth and glucose homeostasis.
Scientific Background: Ribosomal S6 (S6K) belongs to the AGC family of Serine/Threonine protein kinases. There are two forms of S6K, alpha and beta, which have cytoplasmic and nuclear variants derived from alternative splicing (1). The activity of S6K is regulated by phosphorylation/ dephosphorylation events in cellular responses to various extracellular stimuli. Its activity has also been shown to be dependent on nutrient availability (3-6). The mechanism of activation of S6K is a multi-step process which is achieved by phosphorylating the 40S ribosomal protein S6, which then upregulates the translation of mRNA transcripts containing an oligopryimidine tract at the 5' transcriptional start site (3-6). Recently, a highly homologous ribosomal S6 kinase, termed S6K2, was identified. It has 70% amino acid identity in the overall sequence with S6K1, and highly conserved potential phosphorylation sites of S6K1. However, the N- and C-terminal domains of S6K2 are quite different (2). As a result, S6K is a key player in the regulation of cell size, growth and glucose homeostasis.
Product Categories/Family for anti-RPS6KB2 antibody
References
1. Gout, I. et al. (1998). J Biol Chem. 273 (46):30061-30064. 2. Val'Ovka, TI. et al. (2000). Ukr Biokhim Zh. 72(3):31-7. 3. Kawasome, H. et al. (1998) Proc. Natl. Acad. Sci. 95 4. Montagne, J. et al. 1999) Science 285 5. Shima, H. et al. (1998) EMBO J 17: 6649- 6659. 6. Lee-Fruman, K. et al. (1999) Oncogene 18: 5108- 5114.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,455 Da
NCBI Official Full Name
ribosomal protein S6 kinase beta-2
NCBI Official Synonym Full Names
ribosomal protein S6 kinase, 70kDa, polypeptide 2
NCBI Official Symbol
RPS6KB2
NCBI Official Synonym Symbols
KLS; SRK; S6K2; STK14B; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta
NCBI Protein Information
ribosomal protein S6 kinase beta-2; P70S6K2; S6K-beta; p70 S6KB; p70-S6K 2; S6K-beta-2; p70 S6K-beta; p70 S6 kinase beta; S6 kinase-related kinase; p70 ribosomal S6 kinase beta; serine/threonine-protein kinase 14B; 70 kDa ribosomal protein S6 kinase 2; serine/threonine-protein kinase 14 beta
UniProt Protein Name
Ribosomal protein S6 kinase beta-2
Protein Family
UniProt Gene Name
RPS6KB2
UniProt Synonym Gene Names
STK14B; S6K-beta-2; S6K2; P70S6K2; p70-S6K 2; SRK; S6K-beta; p70 S6 kinase beta; p70 S6K-beta; p70 S6KB; p70-beta
UniProt Entry Name
KS6B2_HUMAN
Similar Products
Product Notes
The RPS6KB2 rps6kb2 (Catalog #AAA806789) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p70 S6K Antibody: HRP reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's p70 S6K can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA). 1ug/mL (WB). Researchers should empirically determine the suitability of the RPS6KB2 rps6kb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p70 S6K, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
