Western Blot (WB)
(Western blot analysis of p62 expression in Hela (A), Jurkat (B), mouse liver (C), mouse kidney (D) whole cell lysates.)
Rabbit p62 Polyclonal Antibody | anti-SQSTM1 antibody
Anti-p62 Antibody
Western Blot (WB)
(Western blot analysis of p62 expression in Hela (A), Jurkat (B), mouse liver (C), mouse kidney (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of p62 expression in Hela (A), Jurkat (B), mouse liver (C), mouse kidney (D) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of p62 staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of p62 staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
Uniprot Description
SQSTM1: an adapter protein which binds ubiquitin, shuttling proteins to the proteasome. Co-localizes along with the E3 ubiquitin ligase, TRAF6, to aggregates from Alzheimer's disease brains but not in control brain. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Forms ternary complexes with PKCZ and Kv-beta2 or PKCZ and GABBR3. Also interacts with KCNAB1, GABRR1, GABRR2 and GABRR3. Interacts with EBI3, LCK, RASA1, PKCZ , PKCI, NR2F2, NTRK1, NTRK2, NTRK3, NBR1, MEK5, TRIM55 and MAPKAPK5. Interacts with the proteasome subunits PSMD4 and PSMC2. Interacts with K63- polyubiquitinated MAPT/TAU. Interacts with IKBKB through PRKCZ and PRKCI. Interacts with NGFR through TRAF6 and bridges that complex to NTRK1. Forms a complex with MEK5 and PRKCZ or PRKCI. Component of a ternary complex with PAWR and PRKCZ. Upon TNF-alpha stimulation, interacts with RIPK1 problably bridging IKBKB to the TNF-R1 complex composed of TNF-R1/TNFRSF1A, TRADD and RIPK1. Forms a complex with JUB/Ajuba, PRKCZ and TRAF6. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Autophagy; Ubiquitin conjugating system; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 5q35
Cellular Component: autophagic vacuole; cytoplasm; cytoplasmic vesicle; cytosol; endoplasmic reticulum; inclusion body; late endosome; nucleoplasm; PML body
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein kinase binding; protein kinase C binding; protein serine/threonine kinase activity; receptor tyrosine kinase binding; SH2 domain binding; ubiquitin binding; ubiquitin protein ligase binding; zinc ion binding
Biological Process: apoptosis; autophagy; cell differentiation; endosome transport; immune system process; macroautophagy; mitochondrion degradation; negative regulation of apoptosis; positive regulation of apoptosis; positive regulation of macroautophagy; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; protein heterooligomerization; protein localization; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of Ras protein signal transduction; response to stress; ubiquitin-dependent protein catabolic process
Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 3; Paget Disease Of Bone
Research Articles on SQSTM1
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Product Notes
The SQSTM1 sqstm1 (Catalog #AAA8245349) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-p62 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p62 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1/500 - 1/2000; IHC: 1/50 - 1/200. Researchers should empirically determine the suitability of the SQSTM1 sqstm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p62, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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