Western Blot (WB)
(Western blot analysis of PUMA expression in human K562 cell lysate with anti-PUMA (NT) at 2ug/ml.)
Rabbit anti-Human p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3 Polyclonal Antibody
p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3, NT (PUMA/bbc3)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3; Polyclonal Antibody; NT (PUMA/bbc3); Anti -p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3; anti-p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes a band at ~23kD.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 40% glycerol.
Applicable Applications for anti-p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 2-4ug/ml.
Dilution: Western Blot: 2-4ug/ml.
Immunogen
Raised with a synthetic peptide (ARARQEGSSPEPVEG) corresponding to amino acids 2 to 16 of human PUMA-a (1). This sequence is identical between human and mouse PUMA.
Positive Control
K562 cell lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of PUMA expression in human K562 cell lysate with anti-PUMA (NT) at 2ug/ml.)
Western Blot (WB)
(Western blot analysis of PUMA expression in human K562 cell lysate with anti-PUMA (NT) at 2ug/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of PUMA in human breast carcinoma with anti-PUMA (NT) at 10ug/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of PUMA in human breast carcinoma with anti-PUMA (NT) at 10ug/ml.)
Related Product Information for anti-p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3 antibody
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identi ed recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Product Categories/Family for anti-p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3 antibody
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Product Notes
The p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3 (Catalog #AAA610567) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3, NT (PUMA/bbc3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 2-4ug/ml. Researchers should empirically determine the suitability of the p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p53 Upregulated Modulator of Apoptosis/Bcl 2 Binding Component 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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