Western Blot (WB)
(Western Blot analysis of P4HTM expression in transfected 293T cell line by P4HTM polyclonal antibody. Lane 1: P4HTM transfected lysate (63.1kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human P4HTM Polyclonal Antibody | anti-P4HTM antibody
P4HTM (Transmembrane Prolyl 4-hydroxylase, P4H-TM, Hypoxia-inducible Factor Prolyl Hydroxylase 4, HIF-PH4, HIF-prolyl Hydroxylase 4, HPH-4, PH4, FLJ20262) (Biotin)
Gene Names
P4HTM; PH4; PH-4; PHD4; EGLN4; HIFPH4; P4H-TM
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
P4HTM; Polyclonal Antibody; P4HTM (Transmembrane Prolyl 4-hydroxylase; P4H-TM; Hypoxia-inducible Factor Prolyl Hydroxylase 4; HIF-PH4; HIF-prolyl Hydroxylase 4; HPH-4; PH4; FLJ20262) (Biotin); anti-P4HTM antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human P4HTM.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-P4HTM antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human P4HTM, aa1-563 (NP_808807.2).
Immunogen Sequence
MAAAAVTGQRPETAAAEEASRPQWAPPDHCQAQAAAGLGDGEDAPVRPLCKPRGICSRAYFLVLMVFVHLYLGNVLALLLFVHYSNGDESSDPGPQHRAQGPGPEPTLGPLTRLEGIKVGHERKVQLVTDRDHFIRTLSLKPLLFEIPGFLTDEECRLIIHLAQMKGLQRSQILPTEEYEEAMSTMQVSQLDLFRLLDQNRDGHLQLREVLAQTRLGNGWWMTPESIQEMYAAIKADPDGDGVLSLQEFSNMDLRDFHKYMRSHKAESSELVRNSHHTWLYQGEGAHHIMRAIRQRVLRLTRLSPEIVELSEPLQVVRYGEGGHYHAHVDSGPVYPETICSHTKLVANESVPFETSCRQVSPNWGLPSILRPGTPMTQAQPCTVGVPLGMGPGDHWVIPVSPWEHPQLGTCSVPPLPYSYMTVLFYLNNVTGGGETVFPVADNRTYDEMSLIQDDVDLRDTRRHCDKGNLRVKPQQGTAVFWYNYLPDGQGWVGDVDDYSLHGGCLVTRGTKWIANNWINVDPSRARQALFQQEMARLAREGGTDSQPEWALDRAYRDARVEL
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of P4HTM expression in transfected 293T cell line by P4HTM polyclonal antibody. Lane 1: P4HTM transfected lysate (63.1kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of P4HTM expression in transfected 293T cell line by P4HTM polyclonal antibody. Lane 1: P4HTM transfected lysate (63.1kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-P4HTM antibody
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
Product Categories/Family for anti-P4HTM antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,661 Da
NCBI Official Full Name
transmembrane prolyl 4-hydroxylase isoform c
NCBI Official Synonym Full Names
prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
NCBI Official Symbol
P4HTM
NCBI Official Synonym Symbols
PH4; PH-4; PHD4; EGLN4; HIFPH4; P4H-TM
NCBI Protein Information
transmembrane prolyl 4-hydroxylase; HPH-4; HIF-PH4; proline 4-hydroxylase; HIF-prolyl hydroxylase 4; P4H with transmembrane domain; Prolyl hydroxlase domain-containing 4; hypoxia inducible factor prolyl 4 hydroxylase; hypoxia-inducible factor prolyl 4-hyd
UniProt Protein Name
Transmembrane prolyl 4-hydroxylase
Protein Family
UniProt Gene Name
P4HTM
UniProt Synonym Gene Names
PH4; P4H-TM; HIF-PH4; HIF-prolyl hydroxylase 4; HPH-4
UniProt Entry Name
P4HTM_HUMAN
Similar Products
Product Notes
The P4HTM p4htm (Catalog #AAA6388230) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The P4HTM (Transmembrane Prolyl 4-hydroxylase, P4H-TM, Hypoxia-inducible Factor Prolyl Hydroxylase 4, HIF-PH4, HIF-prolyl Hydroxylase 4, HPH-4, PH4, FLJ20262) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's P4HTM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the P4HTM p4htm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "P4HTM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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