Rabbit anti-Human, Mouse p47Phox Polyclonal Antibody | anti-Neutrophil cytosolic factor 1; NCF1 antibody

p47Phox polyclonal antibody

Cat. Num. AAA565250

• Gene Names: NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Protein A-affinity purified.
• Synonyms: p47Phox; Polyclonal Antibody; p47Phox polyclonal antibody; anti-Neutrophil cytosolic factor 1; NCF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Recognizes human and mouse p47Phox. Detects a band of ~50kDa by Western blot.
• Purity/Purification: Protein A-affinity purified.
• Form/Format: Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 390

• Applicable Applications for anti-Neutrophil cytosolic factor 1; NCF1 antibody: Western Blot (WB)
• Application Notes: Western Blot (1:1,000, ECL); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~50kDa by Western blot.
• Immunogen: Recombinant human p47Phox.
• Preparation and Storage: Long term storage: -20 degree C

Western Blot (WB)

(Western blot analysis of p47phox: Lane 1: MWM, Lane 2: p47Phox (human), (recombinant) (His-tag), Lane 3: RAW 264.7 Cell Lysate.)

Related Product Information for anti-Neutrophil cytosolic factor 1; NCF1 antibody

Originally recognized for their role in the respiratory burst of phagocytes, the NOX family of NADPH oxidases is now known to consist of at least 13 enzymes and enzyme subunits that catalyze the production of superoxide and other downstream reactive oxygen species (ROS) in nearly all cells and tissues. NOX enzymes contribute to a multitude of physiological functions including host defense, cell signaling, and differentiation, with dysregulation linked to pathological manifestations including immunosuppression, hypothyroidism, cardiovascular disease, and neurodegeneration. p47Phox, also known as neutrophil cytosolic factor 1 (NCF1), is considered the organizing subunit of NADPH oxidase enzyme complexes containing the prototypic NOX2 enzyme. Cell stimulation results in phosphorylation of p47Phox, exposing a proline rich region which mediates translocation and association with membrane-associated NOX2/p22 complexes. Translocation of p47Phox is accompanied by the p40Phox subunit and p67Phox activator subunit, which along with GTP-bound Rac completes formation of the active NOX2 oxidase complex. Defects in p47Phox are associated with chronic granulomatous disease (CGD), characterized by an inability of host macrophages and phagocytes to kill ingested microbes, leading to life-threatening infections.

Product Categories/Family for anti-Neutrophil cytosolic factor 1; NCF1 antibody

Protein Synthesis & Folding; Oxidative Stress

NCBI and Uniprot Product Information

• NCBI GI #: 325511390
• NCBI GeneID: 653361
• UniProt Accession #: P14598; O43842; Q2PP07; Q53FR5; Q9BU90; Q9BXI7; Q9BXI8; Q9UDV9; Q9UMU2; A6NEH2; A8K7S9
• Molecular Weight: 30,781 Da
• NCBI Official Full Name: Neutrophil cytosol factor 1
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 1
• NCBI Official Symbol: NCF1
• NCBI Official Synonym Symbols: NCF1A; NOXO2; p47phox; SH3PXD1A
• NCBI Protein Information: neutrophil cytosol factor 1
• UniProt Protein Name: Neutrophil cytosol factor 1
• UniProt Gene Name: NCF1
• UniProt Synonym Gene Names: NOXO2; SH3PXD1A; NCF-1
• UniProt Entry Name: NCF1_HUMAN

NCBI Description

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]

Uniprot Description

p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.

Protein type: EC 1.-.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytosol; extrinsic to membrane; NADPH oxidase complex

Molecular Function: electron carrier activity; phosphatidylinositol-3,4-bisphosphate binding; phosphoinositide binding; protein binding; SH3 domain binding; superoxide-generating NADPH oxidase activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; cell proliferation; cell redox homeostasis; cellular defense response; hydrogen peroxide biosynthetic process; inflammatory response; innate immune response; leukotriene metabolic process; negative regulation of smooth muscle contraction; protein targeting to membrane; respiratory burst; respiratory burst during defense response; response to yeast; small GTPase mediated signal transduction; superoxide metabolic process; superoxide release; vascular endothelial growth factor receptor signaling pathway

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I