Rabbit p40-phox Polyclonal Antibody | anti-p40-phox antibody

p40-phox (R149) Polyclonal Antibody

Cat. Num. AAA3001305

• Gene Names: NCF4; NCF; CGD3; P40PHOX; SH3PXD4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: p40-phox; Polyclonal Antibody; p40-phox (R149) Polyclonal Antibody; Neutrophil cytosol factor 4; NCF-4; Neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; p40phox; NCF4; SH3PXD4; anti-p40-phox antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p40 phox (R149) polyclonal antibody detects endogenous levels of p40 phox protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 339

• Applicable Applications for anti-p40-phox antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 115-170 of Human p40-phox.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p40-phox (R149) pAb at 1:500 dilutionLane1:THP-1 whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:Jurkat whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p40-phox (R149) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-p40-phox antibody

Nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase is a multimeric enzyme system that mediates electron transport from NADPH in the cytoplasm to molecular oxygen in the phagosome, thereby generating reactive oxidant intermediates. Upon neutrophil stimulation, NADPH-oxidase and other cytosolic elements localize to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. There are a number of cytosolic proteins that are involved in NADPH-oxidase activation/deactivation, including p47-phox, p67-phox, p40-phox and the small GTP-binding protein, Rac. Activation of NADPH oxidase is accompanied by the phosphorylation of cytosolic components p40-phox, p47-phox and p67-phox. The PKC consensus phosphorylation sites Thr 154 and Ser 315 in p40-phox are phosphorylated during activation of NADPH oxidase. p40-phox can promote oxidase activation by increasing the affinity of p47-phox for NADPH-oxidase.

NCBI and Uniprot Product Information

• NCBI GI #: 47519798
• NCBI GeneID: 4689
• NCBI Accession #: NP_000622.2
• NCBI GenBank Nucleotide #: NP_000622.2
• UniProt Accession #: Q15080
• Molecular Weight: ~ 39kDa
• NCBI Official Full Name: neutrophil cytosol factor 4 isoform 1
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 4
• NCBI Official Symbol: NCF4
• NCBI Official Synonym Symbols: NCF; CGD3; P40PHOX; SH3PXD4
• NCBI Protein Information: neutrophil cytosol factor 4
• UniProt Protein Name: Neutrophil cytosol factor 4
• UniProt Gene Name: NCF4
• UniProt Synonym Gene Names: SH3PXD4; NCF-4; p40phox
• UniProt Entry Name: NCF4_HUMAN

NCBI Description

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

p40phox: a component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst. Upon neutrophil stimulation, this protein and other cytosolic elements are sent to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. Responsible for the downregulation of NADPH-oxidase. Alternative splicing has been observed.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: membrane; endosome membrane; cytosol; NADPH oxidase complex

Molecular Function: protein dimerization activity; protein binding; superoxide-generating NADPH oxidase activator activity; phosphatidylinositol 3-phosphate binding

Biological Process: positive regulation of catalytic activity; interaction with host; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response; vascular endothelial growth factor receptor signaling pathway

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Iii