Western Blot (WB)
(Western blot analysis of p300 (AcK1542) expression in Jurkat (A), Hela (B) whole cell lysates.)
Rabbit anti-Human, Mouse p300 Polyclonal Antibody | anti-EP300 antibody
Anti-p300 (AcK1542) Acetyl Antibody
Western Blot (WB)
(Western blot analysis of p300 (AcK1542) expression in Jurkat (A), Hela (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of p300 (AcK1542) expression in Jurkat (A), Hela (B) whole cell lysates.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]
Uniprot Description
p300: a histone acetyltransferase and transcriptional co-activator that regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Related to CPB (CREB-binding protein), and like CPB can stimulate transcription through activation of CREB. Specifically inhibited by the adenovirus oncoprotein E1A. A co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Methylated at R580 and R604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates the apoptotic response. Also methylated at R2142 by CARM1, which impairs interaction with NCoA2.
Protein type: Transcription, coactivator/corepressor; Acetyltransferase; Motility/polarity/chemotaxis; EC 2.3.1.48; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 22q13.2
Cellular Component: cytoplasm; histone acetyltransferase complex; nucleoplasm; nucleus; transcription factor complex
Molecular Function: acetyltransferase activity; androgen receptor binding; beta-catenin binding; chromatin binding; chromatin DNA binding; damaged DNA binding; DNA binding; histone acetyltransferase activity; lysine N-acetyltransferase activity; nuclear hormone receptor binding; p53 binding; protein binding; protein C-terminus binding; transcription activator binding; transcription coactivator activity; transcription factor binding; transferase activity, transferring acyl groups; zinc ion binding
Biological Process: apoptosis; B cell differentiation; circadian rhythm; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA repair; establishment and/or maintenance of chromatin architecture; fat cell differentiation; G2/M transition of mitotic cell cycle; gene expression; heart development; innate immune response; internal peptidyl-lysine acetylation; internal protein amino acid acetylation; lung development; mitotic cell cycle; N-terminal peptidyl-lysine acetylation; negative regulation of transcription from RNA polymerase II promoter; nervous system development; Notch signaling pathway; nucleotide-excision repair; organ morphogenesis; platelet formation; positive regulation of gene expression, epigenetic; positive regulation of interferon type I production; positive regulation of protein binding; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription of target genes involved in unfolded protein response; protein amino acid acetylation; protein stabilization; regulation of cell cycle; regulation of gene expression, epigenetic; regulation of transcription, DNA-dependent; response to estrogen stimulus; response to hypoxia; skeletal muscle development; somitogenesis; stimulatory C-type lectin receptor signaling pathway; transcription from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair; viral reproduction
Disease: Colorectal Cancer; Rubinstein-taybi Syndrome 1; Rubinstein-taybi Syndrome 2
Research Articles on EP300
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Product Notes
The EP300 ep300 (Catalog #AAA8241971) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-p300 (AcK1542) Acetyl Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's p300 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: (1/500 - 1/1000). Researchers should empirically determine the suitability of the EP300 ep300 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p300, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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