Western Blot (WB)
(Anti-SELE rabbit monoclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: Mouse liver tissue lysate Lane C: Mouse spleen tissue lysate Lane D: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:67 kDa Observed band size:67 kDa)
Rabbit anti-Mouse E-Selectin Monoclonal Antibody | anti-SELE antibody
Recombinant Anti-E-Selectin Antibody, Rabbit Monoclonal
Gene Names
Sele; Elam; CD62E; E-selectin
Reactivity
Mouse
Applications
Western Blot
Purity
Protein A
Synonyms
E-Selectin; Monoclonal Antibody; Recombinant Anti-E-Selectin Antibody; Rabbit Monoclonal; E-Selectin/CD62e/SELE Antibody; Rabbit MAb; selectin E; Anti-CD62E Antibody; Anti-E-selectin Antibody; Anti-Elam Antibody; anti-SELE antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
015
Specificity
Mouse E-Selectin/CD62e/SELE
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SELE antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse E-Selectin/CD62e/SELE Protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse E-Selectin/CD62e/SELE (rM E-Selectin/CD62e/SELE).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-SELE rabbit monoclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: Mouse liver tissue lysate Lane C: Mouse spleen tissue lysate Lane D: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:67 kDa Observed band size:67 kDa)
Western Blot (WB)
(Anti-SELE rabbit monoclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: Mouse liver tissue lysate Lane C: Mouse spleen tissue lysate Lane D: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:67 kDa Observed band size:67 kDa)
Related Product Information for anti-SELE antibody
E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed on the surfaces of stimulated vascular endothelial cells and is sometimes involved in cancer cell metastasis. E-selectin exhibits a complex mosaic structure consisting of a large extracellular region comprised of a lectin domain, an EGF-like domain, and a short consensus repeat (SCR) domain, followed by a transmembrane region and a relatively short (32 aa) cytoplasmic tail. As a member of the LEC-CAM or selectin family, E-selectin recognises and binds to sialylated carbohydrates including members of the Lewis X and Lewis A families found on monocytes, granulocytes, and T-lymphocytes. E-selectin supports rolling and stable arrest of leukocytes on activated vascular endothelium, and furthermore, it was indicated that it can also transduce an activating stimulus via the MAPK cascade into the endothelial cell during leukocyte adhesion. E-selectin regulates adhesive interactions between certain blood cells and endothelium. The soluble form of E selectin (sE-selectin) is a marker of endothelial activation, and has a potential role in the pathogenesis of cardiovascular disease as raised levels have been found in hypertension, diabetes and hyperlipidemia, although its association in established atherosclerosis disease and its value as a prognostic factor is more controversial. soluble E-selectin is inversely associated with the muscular component of the left ventricle, thereby suggesting that the lack of such a reparative factor may be associated with cardiac remodeling in end-stage renal disease (ESRD) patients. Besides, this adhesion molecule appears to be involved in the pathogenesis of atherosclerosis.
References
Roldn V, et al. (2003) Soluble E-selectin in cardiovascular disease and its risk factors. A review of the literature. Thromb Haemost. 90(6): 1007-20.Kawase J, et al. (2009) Increase in E-selectin expression in umbilical vein endothelial cells by anticancer drugs and inhibition by cimetidine. Oncol Rep. 22(6): 1293-7.Matsumoto K, et al. (2010) Soluble adhesion molecule E-selectin predicts cardiovascular events in Japanese patients with type 2 diabetes mellitus. Metabolism. 59(3): 320-4.Stancanelli B, et al. (2010) Soluble e-selectin is an inverse and independent predictor of left ventricular wall thickness in end-stage renal disease patients. Nephron Clin Pract. 114(1): c74-80.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
66,750 Da
NCBI Official Full Name
E-selectin
NCBI Official Synonym Full Names
selectin, endothelial cell
NCBI Official Symbol
Sele
NCBI Official Synonym Symbols
Elam; CD62E; E-selectin
NCBI Protein Information
E-selectin; ELAM-1; LECAM2; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte-endothelial cell adhesion molecule 2
UniProt Protein Name
E-selectin
Protein Family
UniProt Gene Name
Sele
UniProt Synonym Gene Names
Elam-1; ELAM-1; LECAM2
UniProt Entry Name
LYAM2_MOUSE
Uniprot Description
SELE: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Belongs to the selectin/LECAM family.
Protein type: Membrane protein, integral
Cellular Component: cortical cytoskeleton; extracellular space; membrane; perinuclear region of cytoplasm; integral to membrane; coated pit; caveola; lipid raft
Molecular Function: phospholipase binding; transmembrane receptor activity; carbohydrate binding; sialic acid binding
Biological Process: heterophilic cell adhesion; leukocyte adhesion; phospholipase C activation; positive regulation of leukocyte migration; positive regulation of receptor internalization; actin filament-based process; leukocyte tethering or rolling; cell adhesion; inflammatory response; signal transduction
Research Articles on SELE
Similar Products
Product Notes
The SELE sele (Catalog #AAA8112011) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-E-Selectin Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's E-Selectin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the SELE sele for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E-Selectin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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