P2X5 Polyclonal Antibody | anti-P2X5 antibody

Anti-P2X5 Antibody

Cat. Num. AAA177785

• Gene Names: P2RX5; P2X5; LRH-1; P2X5R
• Reactivity: Mouse, Rat; Predicted to work with: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: P2X5; Polyclonal Antibody; Anti-P2X5 Antibody; P2X purinoceptor 5; ATP receptor; ATP receptor subunit; Ionotropic ATP receptor P2X5; LRH 1; LRH1; Lymphoid restricted histocompatibility antigen 1; MGC47755; P2rx5; P2RX5_HUMAN; P2X5R; Purinergic receptor; Purinergic receptor P2X ligand gated ion channel 5; Purinergic receptor P2X5; purinergic receptor P2X; ligand gated ion channel; 5; anti-P2X5 antibody

• Reactivity: Mouse, Rat; Predicted to work with: Human
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 421

• Applicable Applications for anti-P2X5 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human P2X5 recombinant protein (Position: D333-T422). Human P2X5 shares 42% amino acid (aa) sequence identity with rat P2X5.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- P2X5 Picoband antibody, MBS177785, Western blottingAll lanes: Anti P2X5 (MBS177785) at 0.5ug/mlWB: Rat Brain Tissue Lysate at 50ugPredicted bind size: 47KDObserved bind size: 47KD )

Immunohistochemistry (IHC)

(Anti- P2X5 Picoband antibody, MBS177785, IHC(P)IHC(P): Mouse Brain Tissue )

Immunohistochemistry (IHC)

(Anti- P2X5 Picoband antibody, MBS177785, IHC(P)IHC(P): Rat Brain Tissue )

Related Product Information for anti-P2X5 antibody

Description: Rabbit IgG polyclonal antibody for P2X purinoceptor 5(P2RX5) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: P2X purinoceptor 5 is a protein that in humans is encoded by the P2RX5 gene. The product of this gene belongs to the family of purinoceptors for ATP. It has been identified that the P2RX5 gene within a BAC contig covering chromosome 17p13. This receptor functions as a ligand-gated ion channel. Several characteristic motifs of ATP-gated channels are present in its primary structure, but, unlike other members of the purinoceptors family, this receptor has only a single transmembrane domain. Quantitative dot blots revealed that P2X5R expression is highest in brain and immune system and is developmentally regulated.

References

1. "Entrez Gene: P2RX5 purinergic receptor P2X, ligand-gated ion channel, 5" 2. Le, K.-T., Paquet, M., Nouel, D., Babinski, K., Seguela, P. Primary structure and expression of a naturally truncated human P2X ATP receptor subunit from brain and immune system. FEBS Lett. 418: 195-199, 1997. 3. Touchman, J. W., Anikster, Y., Dietrich, N. L., Maduro, V. V. B., McDowell, G., Shotelersuk, V., Bouffard, G. G., Beckstrom-Sternberg, S. M., Gahl, W. A., Green, E. D. The genomic region encompassing the nephropathic cystinosis gene (CTNS): complete sequencing of a 200-kb segment and discovery of a novel gene within the common cystinosis-causing deletion.Genome Res. 10: 165-173, 2000.

NCBI and Uniprot Product Information

• NCBI GI #: 325197207
• NCBI GeneID: 5026
• NCBI Accession #: NP_001191448.1
• NCBI GenBank Nucleotide #: NM_001204519.1
• UniProt Accession #: Q93086; O43450; O75540; Q308M5; Q59F38; Q8IXW4; Q93087; Q9NZV0; G5E981
• Molecular Weight: 44,531 Da
• NCBI Official Full Name: P2X purinoceptor 5 isoform D
• NCBI Official Synonym Full Names: purinergic receptor P2X 5
• NCBI Official Symbol: P2RX5
• NCBI Official Synonym Symbols: P2X5; LRH-1; P2X5R
• NCBI Protein Information: P2X purinoceptor 5
• UniProt Protein Name: P2X purinoceptor 5
• UniProt Gene Name: P2RX5
• UniProt Synonym Gene Names: P2X5; P2X5
• UniProt Entry Name: P2RX5_HUMAN

NCBI Description

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream gene, TAX1BP3 (Tax1 binding protein 3). [provided by RefSeq, Mar 2011]

Uniprot Description

P2X5: Receptor for ATP that acts as a ligand-gated ion channel. Belongs to the P2X receptor family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: cytoplasm; integral to nuclear inner membrane; integral to plasma membrane; plasma membrane

Molecular Function: ATP binding; ATP-gated cation channel activity; ion channel activity; purinergic nucleotide receptor activity; transmembrane receptor activity

Biological Process: blood coagulation; nervous system development; positive regulation of calcium-mediated signaling; response to ATP; signal transduction; transport