Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using P2RY1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Rabbit P2RY1 Polyclonal Antibody | anti-P2RY1 antibody
P2RY1 Polyclonal Antibody
IHC 1:50 - 1:200
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using P2RY1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using P2RY1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
NCBI and Uniprot Product Information
NCBI Description
The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor functions as a receptor for extracellular ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. [provided by RefSeq, Jul 2008]
Uniprot Description
P2RY1: Receptor for extracellular adenine nucleotides such as ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. Belongs to the G-protein coupled receptor 1 family.
Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR
Chromosomal Location of Human Ortholog: 3q25.2
Cellular Component: postsynaptic membrane; cell surface; mitochondrion; basolateral plasma membrane; integral to plasma membrane; apical plasma membrane; dendrite; plasma membrane
Molecular Function: ATP-activated nucleotide receptor activity; protein binding; purinergic nucleotide receptor activity, G-protein coupled; protein heterodimerization activity; A1 adenosine receptor binding; receptor activity; ADP binding; ATP binding; ADP-activated nucleotide receptor activity
Biological Process: platelet activation; regulation of vasodilation; eating behavior; positive regulation of hormone secretion; positive regulation of inositol trisphosphate biosynthetic process; sensory perception of pain; negative regulation of binding; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; response to mechanical stimulus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of ion transport; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of protein amino acid phosphorylation; regulation of receptor activity; G-protein signaling, adenylate cyclase inhibiting pathway; blood coagulation; adenosine receptor signaling pathway; aging
Research Articles on P2RY1
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Product Notes
The P2RY1 p2ry1 (Catalog #AAA9128907) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The P2RY1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's P2RY1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB 1:500 - 1:2000 IHC 1:50 - 1:200. Researchers should empirically determine the suitability of the P2RY1 p2ry1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "P2RY1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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