Rabbit anti-Human P2RY1 Polyclonal Antibody | anti-P2RY1 antibody

P2RY1, ID (P2RY1, P2Y purinoceptor 1, ATP receptor, Purinergic receptor) (Biotin)

Cat. Num. AAA6330254

• Gene Names: P2RY1; P2Y1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: P2RY1; Polyclonal Antibody; ID (P2RY1; P2Y purinoceptor 1; ATP receptor; Purinergic receptor) (Biotin); Purinergic receptor; anti-P2RY1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-P2RY1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: P2RY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-260 amino acids from the Central region of human P2RY1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(P2RY1 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the P2RY1 antibody detected the P2RY1 protein (arrow).)

Related Product Information for anti-P2RY1 antibody

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor functions as a receptor for extracellular ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. [provided by RefSeq].

Product Categories/Family for anti-P2RY1 antibody

Antibodies; Receptors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Maloney, S.F., et al. Integr Biol (Camb) 2(4):183-192(2010). Nisar, S., et al. Traffic 11(4):508-519(2010). Kumar, T.S., et al. J. Med. Chem. 53(6):2562-2576(2010). Bambace, N.M., et al. Platelets 21(2):85-93(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4505557
• NCBI GeneID: 5028
• NCBI Accession #: NP_002554.1
• NCBI GenBank Nucleotide #: NM_002563.3
• UniProt Accession #: P47900
• Molecular Weight: 42,072 Da
• NCBI Official Full Name: P2Y purinoceptor 1
• NCBI Official Synonym Full Names: purinergic receptor P2Y, G-protein coupled, 1
• NCBI Official Symbol: P2RY1
• NCBI Official Synonym Symbols: P2Y1
• NCBI Protein Information: P2Y purinoceptor 1; ATP receptor; platelet ADP receptor; P2 purinoceptor subtype Y1
• UniProt Protein Name: P2Y purinoceptor 1
• Protein Family: P2Y purinoceptor
• UniProt Gene Name: P2RY1
• UniProt Synonym Gene Names: P2Y1
• UniProt Entry Name: P2RY1_HUMAN

NCBI Description

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor functions as a receptor for extracellular ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. [provided by RefSeq, Jul 2008]

Uniprot Description

P2RY1: Receptor for extracellular adenine nucleotides such as ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q25.2

Cellular Component: postsynaptic membrane; cell surface; mitochondrion; basolateral plasma membrane; integral to plasma membrane; dendrite; apical plasma membrane; plasma membrane

Molecular Function: ATP-activated nucleotide receptor activity; protein binding; purinergic nucleotide receptor activity, G-protein coupled; protein heterodimerization activity; A1 adenosine receptor binding; receptor activity; ADP binding; ATP binding; ADP-activated nucleotide receptor activity

Biological Process: platelet activation; regulation of vasodilation; eating behavior; positive regulation of hormone secretion; positive regulation of inositol trisphosphate biosynthetic process; negative regulation of binding; sensory perception of pain; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; response to mechanical stimulus; positive regulation of ion transport; positive regulation of transcription from RNA polymerase II promoter; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of receptor activity; G-protein signaling, adenylate cyclase inhibiting pathway; positive regulation of protein amino acid phosphorylation; blood coagulation; adenosine receptor signaling pathway; aging