Human Hemoglobin Delta (HBd) ELISA Kit | HBd elisa kit

Human Hemoglobin Delta (HBd) ELISA Kit

Cat. Num. AAA2700602

• Reactivity: Human
• Synonyms: Hemoglobin Delta (HBd); Human Hemoglobin Delta (HBd) ELISA Kit; Delta-globin; HBd elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hemoglobin Delta (HBd).; No significant cross-reactivity or interference between Hemoglobin Delta (HBd) and analogues was observed.
• Sequence Length: 147

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Erythrocyte Lysates and Other Biological Fluids
• Detection Range: 2.47-200ug/mL
• Sensitivity: < 0.91ug/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemoglobin Delta (HBd) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemoglobin Delta (HBd) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HBd elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hemoglobin Delta (HBd) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hemoglobin Delta (HBd) and unlabeled Hemoglobin Delta (HBd) (Standards or samples) with the pre-coated antibody specific to Hemoglobin Delta (HBd). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hemoglobin Delta (HBd) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hemoglobin Delta (HBd) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 4504351
• NCBI GeneID: 3045
• NCBI Accession #: NP_000510.1
• NCBI GenBank Nucleotide #: NM_000519.3
• UniProt Accession #: P02042; Q3Y5H3; Q8WXT7
• Molecular Weight: 16,055 Da
• NCBI Official Full Name: hemoglobin subunit delta
• NCBI Official Synonym Full Names: hemoglobin subunit delta
• NCBI Official Symbol: HBD
• NCBI Protein Information: hemoglobin subunit delta
• UniProt Protein Name: Hemoglobin subunit delta
• Protein Family: Hemoglobin
• UniProt Gene Name: HBD

NCBI Description

The delta (HBD) and beta (HBB) genes are normally expressed in the adult: two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin. Two alpha chains plus two delta chains constitute HbA-2, which with HbF comprises the remaining 3% of adult hemoglobin. Five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon--Ggamma--Agamma--delta--beta-3'. Mutations in the delta-globin gene are associated with beta-thalassemia. [provided by RefSeq, Jul 2008]

Uniprot Description

HBD: Involved in oxygen transport from the lung to the various peripheral tissues. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: blood microparticle; cytosol; hemoglobin complex

Molecular Function: heme binding; iron ion binding; oxygen binding; oxygen carrier activity; protein binding

Biological Process: blood coagulation; oxygen transport