Immunohistochemistry (IHC)
(Formalin-Fixed Paraffin Embedded (FFPE) 313 cell pellet was stained with Rabbit Antip18INK4C antibody at 1 :200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Rabbit p18INK4C Polyclonal Antibody | anti-p18INK4C antibody
Rabbit anti p18INK4C Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Epitope Affinity Purification
Synonyms
p18INK4C; Polyclonal Antibody; Rabbit anti p18INK4C Polyclonal Antibody; Cyclin-dependent kinase inhibitor 2C; CDKN2C; P18; P18INK4C; anti-p18INK4C antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody recognizes p18INK4C protein. It crossreacts to human, mouse and rat. Others are not tested.
Purity/Purification
Purified by Epitope Affinity Purification
Form/Format
Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-p18INK4C antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:200
Flow Cytometry: Not tested
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:200
Flow Cytometry: Not tested
Antigen Preparation
A synthetic peptide corresponding to the C-terminus of Mouse p18INK4G. This sequence is identical to human, rat and mouse.
Positive Control
3T3, Hela
Cellular Location
Nuclear
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C - 8 degree C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles. Optimal dilutions should be determined by researchers for the specific applications.
Immunohistochemistry (IHC)
(Formalin-Fixed Paraffin Embedded (FFPE) 313 cell pellet was stained with Rabbit Antip18INK4C antibody at 1 :200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Immunohistochemistry (IHC)
(Formalin-Fixed Paraffin Embedded (FFPE) 313 cell pellet was stained with Rabbit Antip18INK4C antibody at 1 :200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Related Product Information for anti-p18INK4C antibody
Background:
Cyclin-dependent kinases (CDKs) are activated in part by forming complexes with cyclins. For example, CDK4 and CDK6 associate with the D-type cyclins ,md phosphorylate the retinoblastoma protein. This phosphorylation is a necessary event for cells to enter S-phase. The inhibitors of CDK4 (INK4) family include p151NK4B, p16 INK4A, p181NK4C and p19INK4D. p18 has been shown to function as a aploinsufficient tumor suppressor in vivo. All INK4 proteins are composed of 32 amino acid ankyrin motifs and selectively inhibit CDK4/6 activity. Mutational analyses of p18 implicate the third and the aminoterminal portion of the fourth ankyrin repeat in mediating binding to CDK4/6. The interaction of INK4 family members can be a binary complex with CDK4/6 or ternary complex with cyclin D-bound CDK4/6 and ultimately results in the inhibition of cell cycle progression. The gene encoding p15INK4B is often silenced by hypermethylation in acute myeloid leukemia and deleted in acute lymphocytic leukemia.
Cyclin-dependent kinases (CDKs) are activated in part by forming complexes with cyclins. For example, CDK4 and CDK6 associate with the D-type cyclins ,md phosphorylate the retinoblastoma protein. This phosphorylation is a necessary event for cells to enter S-phase. The inhibitors of CDK4 (INK4) family include p151NK4B, p16 INK4A, p181NK4C and p19INK4D. p18 has been shown to function as a aploinsufficient tumor suppressor in vivo. All INK4 proteins are composed of 32 amino acid ankyrin motifs and selectively inhibit CDK4/6 activity. Mutational analyses of p18 implicate the third and the aminoterminal portion of the fourth ankyrin repeat in mediating binding to CDK4/6. The interaction of INK4 family members can be a binary complex with CDK4/6 or ternary complex with cyclin D-bound CDK4/6 and ultimately results in the inhibition of cell cycle progression. The gene encoding p15INK4B is often silenced by hypermethylation in acute myeloid leukemia and deleted in acute lymphocytic leukemia.
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Product Notes
The p18INK4C (Catalog #AAA460113) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti p18INK4C Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p18INK4C can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 1:200 Flow Cytometry: Not tested. Researchers should empirically determine the suitability of the p18INK4C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p18INK4C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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