Western Blot (WB)
(Western blot analysis of extracts from K562 cells (Lane 2) and 3T3 cells (Lane 3), using P130 Cas(Phospho-Tyr410) Antibody. The lane on the left is treated with antigen-specific peptide.)
Rabbit anti-Human p130 Cas Polyclonal Antibody | anti-p130 Cas antibody
p130 Cas (Phospho-Tyr410) Antibody
Gene Names
BCAR1; CAS; CAS1; CASS1; CRKAS; P130Cas
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Synonyms
p130 Cas; Polyclonal Antibody; p130 Cas (Phospho-Tyr410) Antibody; BCAR1; CRKAS; CRK-ASSOCIATED SUBSTRATE; anti-p130 Cas antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of p130 Cas only when phosphorylated at tyrosine 410.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
916
Applicable Applications for anti-p130 Cas antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunohistochemistry: 1:50~1:100
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of tyrosine 410(G-V-Y(p)-A-V) derived from Human p130 Cas.
Modification
Phospho-Tyr410
Target Name
p130 Cas
Preparation and Storage
Store at -20 degree C/1 year
Western Blot (WB)
(Western blot analysis of extracts from K562 cells (Lane 2) and 3T3 cells (Lane 3), using P130 Cas(Phospho-Tyr410) Antibody. The lane on the left is treated with antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells (Lane 2) and 3T3 cells (Lane 3), using P130 Cas(Phospho-Tyr410) Antibody. The lane on the left is treated with antigen-specific peptide.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p130 Cas (Phospho-Tyr410) antibody (left)or the same antibody preincubated with blocking peptide (right).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p130 Cas (Phospho-Tyr410) antibody (left)or the same antibody preincubated with blocking peptide (right).)
Related Product Information for anti-p130 Cas antibody
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.Katarzyna Modzelewska, J. Biol. Chem., Dec 2006; 281: 37527 - 37535.Kevin Ogden, Am J Physiol Heart Circ Physiol, Dec 2006; 291: H2857 - H2863.Sangkil Nam, Cancer Res., Oct 2005; 65: 9185 - 9189.Norikazu Yamana, Mol. Cell. Biol., Sep 2006; 26: 6844 - 6
Product Categories/Family for anti-p130 Cas antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
93,291 Da
NCBI Official Full Name
breast cancer anti-estrogen resistance protein 1 isoform 1
NCBI Official Synonym Full Names
breast cancer anti-estrogen resistance 1
NCBI Official Symbol
BCAR1
NCBI Official Synonym Symbols
CAS; CAS1; CASS1; CRKAS; P130Cas
NCBI Protein Information
breast cancer anti-estrogen resistance protein 1
UniProt Protein Name
Breast cancer anti-estrogen resistance protein 1
UniProt Gene Name
BCAR1
UniProt Synonym Gene Names
CAS; CASS1; CRKAS
UniProt Entry Name
BCAR1_HUMAN
NCBI Description
BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009]
Uniprot Description
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
Research Articles on p130 Cas
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Product Notes
The p130 Cas bcar1 (Catalog #AAA9403264) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p130 Cas (Phospho-Tyr410) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p130 Cas can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100. Researchers should empirically determine the suitability of the p130 Cas bcar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p130 Cas, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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