Testing Data
Rabbit IgG P-glycoprotein Polyclonal Antibody | anti-ABCB1 antibody
P-glycoprotein Antibody
Other applications are possible but have not been tested.
*Note: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for staining formalin/paraffin sections.
**Note: This information is only intended as a quide. The optimal dilutions must be determined by the user.
Testing Data
Testing Data
Background: P-gp, also called MDR1 or ABCB1, is a glycoprotein that in humans is encoded by the ABCB1 gene. P-gp is a well-characterized ABC-transporter (which transports a wide variety of xenobiotics across extra- and intracellular membranes). It is an ATP-dependent drug efflux pump with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein functions in the kidneys, liver, blood-brain barrier and the intestinal epithelium.
NCBI and Uniprot Product Information
NCBI Description
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq, Jul 2008]
Uniprot Description
ABCB1: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. Interacts with PSMB8.
Protein type: Cell adhesion; Transporter, ABC family; Hydrolase; EC 3.6.3.44; Membrane protein, integral; Transporter; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 7q21.12
Cellular Component: cell surface; membrane; integral to membrane; plasma membrane
Molecular Function: protein binding; ATPase activity, coupled to transmembrane movement of substances; transporter activity; ATP binding; xenobiotic-transporting ATPase activity
Biological Process: response to drug; transport; multidrug transport; xenobiotic transport; G2/M transition of mitotic cell cycle; transmembrane transport
Disease: Colchicine Resistance; Inflammatory Bowel Disease 13
Research Articles on ABCB1
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Product Notes
The ABCB1 abcb1 (Catalog #AAA840947) is an Antibody produced from Rabbit IgG and is intended for research purposes only. The product is available for immediate purchase. The P-glycoprotein Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's P-glycoprotein can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin/Paraffin. We suggest starting with 0.5-1ug/ml** for IHC-P* or IHC-F. Other applications are possible but have not been tested. *Note: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for staining formalin/paraffin sections. **Note: This information is only intended as a quide. The optimal dilutions must be determined by the user. Researchers should empirically determine the suitability of the ABCB1 abcb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "P-glycoprotein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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