Rabbit anti-Mouse, Human Oxsr1 Polyclonal Antibody | anti-Oxsr1 antibody

Oxsr1, NT (Oxsr1, Osr1, Serine/threonine-protein kinase OSR1, Oxidative stress-responsive 1 protein) (FITC)

Cat. Num. AAA6330046

• Gene Names: Oxsr1; Osr1; AI462649; AW209236; mKIAA1101; 2210022N24Rik; 2810422B09Rik
• Reactivity: Mouse, Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Oxsr1; Polyclonal Antibody; NT (Oxsr1; Osr1; Serine/threonine-protein kinase OSR1; Oxidative stress-responsive 1 protein) (FITC); Oxidative stress-responsive 1 protein; anti-Oxsr1 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Oxsr1. Species Crossreactivity: human
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 471

• Applicable Applications for anti-Oxsr1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 94-121 in the N-terminal region of mouse Oxsr1, UniProt Accession #NP_598746.1; Q6P9R2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6004178. This demonstrates that MBS6004178 detected the Oxsr1 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in 293 cell lysates (2 ug/lane) usingMBS6004178, either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Oxsr1 gene.)

Western Blot (WB)

(Western Blot analysis in mouse stomach tissue lysates (35ug/lane) usingMBS6004178. This demonstrates that MBS6004178 detected the Oxsr1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse duodenum tissue using MBS6004178 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6004178 for immunohistochemistry.)

Related Product Information for anti-Oxsr1 antibody

Oxsr1 regulates downstream kinases in response to environmental stress. May also have a function in regulating the actin cytoskeleton.

Product Categories/Family for anti-Oxsr1 antibody

Antibodies; Protein Kinases

References

1. Hengl, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(13):6052-6057(2010). 2. Geng, Y., et al. J. Biol. Chem. 284(21):14020-14028(2009). 3. Vallon, V. Kidney Int. 74(11):1373-1375(2008). 4. Chiga, M., et al. Kidney Int. 74(11):1403-1409(2008). 5. Gagnon, K.B., et al. Mol. Cell. Biol. 26(2):689-698(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 110626165
• NCBI GeneID: 108737
• NCBI Accession #: NP_598746.1
• NCBI GenBank Nucleotide #: NP_598746.1
• UniProt Accession #: Q6P9R2
• NCBI Official Full Name: serine/threonine-protein kinase OSR1
• NCBI Official Synonym Full Names: oxidative-stress responsive 1
• NCBI Official Symbol: Oxsr1
• NCBI Official Synonym Symbols: Osr1; AI462649; AW209236; mKIAA1101; 2210022N24Rik; 2810422B09Rik
• NCBI Protein Information: serine/threonine-protein kinase OSR1
• UniProt Protein Name: Serine/threonine-protein kinase OSR1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Oxsr1
• UniProt Synonym Gene Names: Osr1
• UniProt Entry Name: OXSR1_MOUSE