Rabbit Nibrin Polyclonal Antibody | anti-S343 antibody

Nibrin (Phospho-S343) Polyclonal Antibody

Cat. Num. AAA3004751

• Gene Names: NBN; ATV; NBS; P95; NBS1; AT-V1; AT-V2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Nibrin; Polyclonal Antibody; Nibrin (Phospho-S343) Polyclonal Antibody; Nijmegen breakage syndrome protein 1; Cell cycle regulatory protein p95; NBS1/P95; NBS; NBS1; P95; anti-S343 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-p95/NBS1 (S343) polyclonal antibody detects endogenous levels of p95/NBS1 protein when phosphorylated at Ser343.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 754

• Applicable Applications for anti-S343 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human Nibrin around the phosphorylation site of Serine 343.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-Nibrin (S343)  pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with UV for 5 minutes then repair for 1 hours whole cell lysate (40ug) Lane3:HEK293T treated with UV for 5 minutes then repair for 3 hours whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-p95/NBS1 (S343) pAb in paraffin-embedded human tonsil tissue.)

Related Product Information for anti-S343 antibody

The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex (MRN complex). This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions. In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. NBS1 is expressed ubiquitously and presents high levels in testis.

NCBI and Uniprot Product Information

• NCBI GI #: 33356172
• NCBI GeneID: 4683
• NCBI Accession #: NP_002476.2
• NCBI GenBank Nucleotide #: NP_002476.2
• UniProt Accession #: O60934
• Molecular Weight: ~ 95kDa
• NCBI Official Full Name: nibrin isoform 1
• NCBI Official Synonym Full Names: nibrin
• NCBI Official Symbol: NBN
• NCBI Official Synonym Symbols: ATV; NBS; P95; NBS1; AT-V1; AT-V2
• NCBI Protein Information: nibrin
• UniProt Protein Name: Nibrin
• Protein Family: Nibrin
• UniProt Gene Name: NBN
• UniProt Synonym Gene Names: NBS; NBS1; P95
• UniProt Entry Name: NBN_HUMAN

NCBI Description

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]

Uniprot Description

NBS1: a member of the MRE11/RAD50 double-strand break repair complex. Involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Mutation results in the Nijmegen breakage syndrome (NBS), an autosomal recessive chromosomal instability syndrome.

Protein type: Cell cycle regulation; DNA repair, damage

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: nucleoplasm; PML body; Mre11 complex; nuclear chromosome, telomeric region; nucleolus; replication fork; nucleus; nuclear inclusion body

Molecular Function: ATP-dependent DNA helicase activity; protein binding; damaged DNA binding; protein N-terminus binding; transcription factor binding

Biological Process: DNA damage response, signal transduction by p53 class mediator; mitotic cell cycle checkpoint; positive regulation of kinase activity; isotype switching; DNA repair; positive regulation of protein amino acid autophosphorylation; DNA duplex unwinding; double-strand break repair via homologous recombination; regulation of DNA replication initiation; cell proliferation; meiotic cell cycle; double-strand break repair; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA damage checkpoint; blastocyst growth; cell cycle arrest; neuromuscular process controlling balance; telomere maintenance

Disease: Leukemia, Acute Lymphoblastic; Nijmegen Breakage Syndrome; Aplastic Anemia