Rabbit anti-Human OTUD3 Polyclonal Antibody | anti-OTUD3 antibody

OTUD3, ID (OTUD3, KIAA0459, OTU domain-containing protein 3) (APC)

Cat. Num. AAA6329901

• Gene Names: OTUD3; DUBA4; RP11-460G22.1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: OTUD3; Polyclonal Antibody; ID (OTUD3; KIAA0459; OTU domain-containing protein 3) (APC); OTU domain-containing protein 3; anti-OTUD3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-OTUD3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: OTUD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-220 amino acids from the Central region of human OTUD3.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(OTUD3 Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the OTUD3 antibody detected the OTUD3 protein (arrow).)

Product Categories/Family for anti-OTUD3 antibody

Antibodies; KIAA; orf

References

McGovern, D.P., et al. Nat. Genet. 42(4):332-337(2010). Franke, A., et al. Nat. Genet. 42(4):292-294(2010). Barrett, J.C., et al. Nat. Genet. 41(12):1330-1334(2009). Silverberg, M.S., et al. Nat. Genet. 41(2):216-220(2009). Venter, J.C., et al. Science 291(5507):1304-1351(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 149192871
• NCBI GeneID: 23252
• NCBI Accession #: NP_056022.1
• NCBI GenBank Nucleotide #: NM_015207.1
• UniProt Accession #: Q5T2D3; O75047
• Molecular Weight: 45,124 Da
• NCBI Official Full Name: OTU domain-containing protein 3
• NCBI Official Synonym Full Names: OTU domain containing 3
• NCBI Official Symbol: OTUD3
• NCBI Official Synonym Symbols: DUBA4; RP11-460G22.1
• NCBI Protein Information: OTU domain-containing protein 3
• UniProt Protein Name: OTU domain-containing protein 3
• Protein Family: OTU domain-containing protein
• UniProt Gene Name: OTUD3
• UniProt Synonym Gene Names: KIAA0459
• UniProt Entry Name: OTUD3_HUMAN

Uniprot Description

Function: Deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. Also hydrolyzes heterotypic (mixed and branched) and homotypic chains. Ref.4

Catalytic activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.4

Domain: The UBA-like domain has no influence on ubiquitin hydrolysis (Ref.4).Specificity is given by the S1' ubiquitin-binding site within the OTU domain composed of the Cys-, His- and Variable-loops (Ref.4).

Sequence similarities: Contains 1 OTU domain.Contains 1 UBA-like domain.

Sequence caution: The sequence BAA32304.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.