Western Blot (WB)
(OTOP1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the OTOP1 antibody detected the OTOP1 protein (arrow).)
Rabbit anti-Mouse OTOP1 Polyclonal Antibody | anti-OTOP1 antibody
OTOP1, ID (OTOP1, Otopetrin-1) (FITC)
Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
OTOP1; Polyclonal Antibody; ID (OTOP1; Otopetrin-1) (FITC); Otopetrin-1; anti-OTOP1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-OTOP1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
OTOP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 367-396 amino acids from the Central region of human OTOP1.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(OTOP1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the OTOP1 antibody detected the OTOP1 protein (arrow).)
Western Blot (WB)
(OTOP1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the OTOP1 antibody detected the OTOP1 protein (arrow).)
Immunohistochemistry (IHC)
(OTOP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OTOP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(OTOP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OTOP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-OTOP1 antibody
OTOP1 is required for normal formation of otoconia in the inner ear. Inhibits P2Y purinoceptors. Modulates calcium homeostasis and influx of calcium in response to extracellular ATP (By similarity).
Product Categories/Family for anti-OTOP1 antibody
References
Hurle, B., et al. Hum. Mol. Genet. 12(7):777-789(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,353 Da
NCBI Official Full Name
otopetrin-1
NCBI Official Synonym Full Names
otopetrin 1
NCBI Official Symbol
OTOP1
NCBI Protein Information
otopetrin-1
UniProt Protein Name
Otopetrin-1
Protein Family
UniProt Gene Name
OTOP1
UniProt Entry Name
OTOP1_HUMAN
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Product Notes
The OTOP1 otop1 (Catalog #AAA6329826) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The OTOP1, ID (OTOP1, Otopetrin-1) (FITC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's OTOP1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the OTOP1 otop1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OTOP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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