Rabbit anti-Human OTOL1 Polyclonal Antibody | anti-OTOL1 antibody

OTOL1, NT (OTOL1, Otolin-1) (AP)

Cat. Num. AAA6329812

• Gene Names: OTOL1; C1QTNF15; C1QTNF16
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: OTOL1; Polyclonal Antibody; NT (OTOL1; Otolin-1) (AP); Otolin-1; anti-OTOL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OTOL1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-OTOL1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 11-39 in the N-terminal region of human OTOL1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H292 cell line lysates (35ug/lane) usingMBS6005685. This demonstrates that MBS6005685 detected the OTOL1 protein (arrow).)

Product Categories/Family for anti-OTOL1 antibody

Antibodies; Proteins

References

1. Lasky-Su, J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1345-1354 (2008). 2. Ghai, R., et al. Immunobiology 212 (4-5), 253-266 (2007). 3. Murayama, E., et al. Mech. Dev. 122(6):791-803(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 122937273
• NCBI GeneID: 131149
• NCBI Accession #: NP_001073909.1
• NCBI GenBank Nucleotide #: NM_001080440.1
• UniProt Accession #: A6NHN0
• Molecular Weight: 49,422 Da
• NCBI Official Full Name: otolin-1
• NCBI Official Synonym Full Names: otolin 1
• NCBI Official Symbol: OTOL1
• NCBI Official Synonym Symbols: C1QTNF15; C1QTNF16
• NCBI Protein Information: otolin-1
• UniProt Protein Name: Otolin-1
• Protein Family: Otolin
• UniProt Gene Name: OTOL1

NCBI Description

This gene encodes a secreted glycoprotein with a C-terminal complement Cq1-like globular domain that belongs to the C1q/tumor necrosis factor-related protein (CTRP) family. The encoded protein is expressed in the inner ear and forms a multimeric complex called the otoconia, together with cerebellin-1 and otoconin-90, as part of the otoconial membrane. It contains extensive posttranslational modifications including hydroxylated prolines and glycosylated lysines. Naturally occurring mutations in this gene are associated with abnormal otoconia formation and balance deficits resulting from vestibular dysfunction. [provided by RefSeq, Jul 2017]

Uniprot Description

Collagen-like protein specifically expressed in the inner ear, which provides an organic scaffold for otoconia, a calcium carbonate structure in the saccule and utricle of the ear. Acts as a scaffold for biomineralization: sequesters calcium and forms interconnecting fibrils between otoconia that are incorporated into the calcium crystal structure. Together with OC90, modulates calcite crystal morphology and growth kinetics.