Western Blot (WB)
(Western Blot analysis of SPP1 expression in transfected 293T cell line by SPP1 polyclonal antibody. Lane 1: SPP1 transfected lysate (35.4kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human Osteopontin Polyclonal Antibody | anti-OPN antibody
Osteopontin (Bone Sialoprotein 1, Nephropontin, Secreted Phosphoprotein 1, SPP-1, Urinary Stone Protein, Uropontin, SPP1, BNSP, OPN, PSEC0156, MGC110940) (AP)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Osteopontin; Polyclonal Antibody; Osteopontin (Bone Sialoprotein 1; Nephropontin; Secreted Phosphoprotein 1; SPP-1; Urinary Stone Protein; Uropontin; SPP1; BNSP; OPN; PSEC0156; MGC110940) (AP); anti-OPN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SPP1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Sequence Length
314
Applicable Applications for anti-OPN antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human SPP1, aa1-314 (AAH17387.1).
Immunogen Sequence
MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of SPP1 expression in transfected 293T cell line by SPP1 polyclonal antibody. Lane 1: SPP1 transfected lysate (35.4kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of SPP1 expression in transfected 293T cell line by SPP1 polyclonal antibody. Lane 1: SPP1 transfected lysate (35.4kD). Lane 2: Non-transfected lysate.)
Testing Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between SPP1 and F2. HeLa cells were stained with SPP1 rabbit purified polyclonal 1:1200 and F2 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Testing Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between SPP1 and F2. HeLa cells were stained with SPP1 rabbit purified polyclonal 1:1200 and F2 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Related Product Information for anti-OPN antibody
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Product Categories/Family for anti-OPN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Secreted phosphoprotein 1
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
SPP1
NCBI Official Synonym Symbols
OPN; BNSP; BSPI; ETA-1
NCBI Protein Information
osteopontin
Protein Family
NCBI Description
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Research Articles on OPN
Similar Products
Product Notes
The OPN (Catalog #AAA6388118) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Osteopontin (Bone Sialoprotein 1, Nephropontin, Secreted Phosphoprotein 1, SPP-1, Urinary Stone Protein, Uropontin, SPP1, BNSP, OPN, PSEC0156, MGC110940) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Osteopontin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the OPN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Osteopontin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
