Rabbit anti-Human, Rat Osteoblast Specific Factor 2 Polyclonal Antibody | anti-OSF-2 antibody

Osteoblast Specific Factor 2 (OSF-2, Periostin, Fasciclin-I like) (Azide free) (HRP)

Cat. Num. AAA6491182

• Gene Names: POSTN; PN; OSF2; OSF-2; PDLPOSTN; periostin
• Reactivity: Human, Rat
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Osteoblast Specific Factor 2; Polyclonal Antibody; Osteoblast Specific Factor 2 (OSF-2; Periostin; Fasciclin-I like) (Azide free) (HRP); anti-OSF-2 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Osteoblast Specific Factor 2. Species Crossreactivity: rat
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-OSF-2 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 687-716 in the C-terminal region of human Osteoblast Specific Factor 2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. HRP conjugates are sensitive to light.

Western Blot (WB)

(Western Blot analysis in MCF-7 (lane 1), A375 (lane 2) cell line lysates (35ug/lane) using O8057-07. This demonstrates that O8057-07 detected the Osteoblast Specific Factor 2 protein (arrow).)

Western Blot (WB)

(Western Blot using O8057-07 (1:1000) Lane 1: human breast lysate Lane 2: rat lung lysate Lysates/proteins at 20ug/lane. Secondary IgG (HRP)Goat Anti-Rabbit at 1:10,000. Predicted band size: 93kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue using O8057-07 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of O8057-07 for immunohistochemistry.)

Product Categories/Family for anti-OSF-2 antibody

Antibodies; Growth Factors; Cytokines

References

1. Horiuchi K, Amizuka N, Takeshita S, Takamatsu H, Katsuura M, Ozawa H, Toyama Y, Bonewald LF, Kudo A. J Bone Miner Res. 1999; 14: 1239-49. 2. Takeshita S, Kikuno R, Tezuka K, Amann E. Biochem J. 1993; 294 (Pt1): 271-8. 3. Ulstrup JC, Jeansson S, Wiker HG, Harboe M. Infect Immun. 1995; 63: 672-5.

NCBI and Uniprot Product Information

• NCBI GI #: 209862907
• NCBI GeneID: 10631
• NCBI Accession #: NP_006466
• NCBI GenBank Nucleotide #: NM_006475
• UniProt Accession #: Q15063; Q15064; Q29XZ0; Q3KPJ5; Q5VSY5; Q8IZF9; B1ALD8; C0IMJ1; C0IMJ2; C0IMJ4; D2KRH7; F5H628
• Molecular Weight: 80,357 Da
• NCBI Official Full Name: periostin isoform 1
• NCBI Official Synonym Full Names: periostin, osteoblast specific factor
• NCBI Official Symbol: POSTN
• NCBI Official Synonym Symbols: PN; OSF2; OSF-2; PDLPOSTN; periostin
• NCBI Protein Information: periostin; osteoblast specific factor 2 (fasciclin I-like); periodontal ligament-specific periostin
• UniProt Protein Name: Periostin
• UniProt Gene Name: POSTN
• UniProt Synonym Gene Names: OSF2; PN; OSF-2
• UniProt Entry Name: POSTN_HUMAN

Uniprot Description

POSTN: Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Cell adhesion; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q13.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; trans-Golgi network

Molecular Function: heparin binding; protein binding; cell adhesion molecule binding

Biological Process: tissue development; extracellular matrix organization and biogenesis; regulation of Notch signaling pathway; cell adhesion; skeletal development